BDNFNCT07189130
The Acute Effect of Different Resistance Exercise Loads on Irisin and Brain-derived Neurotrophic Factor in Blood Serum and Plasma
A study testing Exercise: 80% 1 RM with 2 minutes break, Exercise: 80% 1RM with1 minute break and Exercise: accenuated eccentric exercise in people with BDNF and Irisin, run by University of Ljubljana at 1 site in Slovenia.
Trial summary
The aim of this study is to investigate different resistance training loads on BNDF and irisin levels in platelets, blood serum and plasma. Read more about this study
- Who is it for?
- Ages 18 to 30 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- BDNF, Irisin, Resistance Exercise
- What is being tested?
- Exercise: 80% 1 RM with 2 minutes break, Exercise: 80% 1RM with1 minute break and Exercise: accenuated eccentric exercise
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 24 participants
- Where
- 1 siteSlovenia
- Study dates
- 12 May 2025to Sep 2026
- Phase
- Interventional
- Are there results?
- Not yet: the study is under way
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 30 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- males between 18 and 30 years
- moderate level of physical activity assessed with IPAQ
Who cannot join
- injuries
- cardiovascular diseases
- respiratory diseases
- psychiatric diseases
- metabolic diseases
- other neurodegenerative diseases
Trial locations and contacts
1 site in 1 country.
About this study
Official title: The Acute Response of Irisin and Brain-derived Neurotrophic Factor in Blood to Resistance Exercise Load
The aim of this study is to investigate different resistance training loads on BNDF and irisin levels in platelets, blood serum and plasma.
Read the detailed description
In this study, the investigators will examine how three resistance training protocols acutely affect BDNF levels (in serum, plasma, platelet count, and BDNF per platelet) and irisin levels in blood (plasma). The resistance training protocols will differ in intensity, rest intervals between sets and repetition execution. Blood samples will be collected from participants at four time points: before the start of the protocol, 5 minutes after completion of the final set, 1 hour after completion of the final set, and 3 hours after completion of the final set. In addition, blood lactate levels will be monitored at the same time points. Heart rate will also be recorded (immediately before the start of the protocol and 30 seconds after completion of the final set). A control protocol will also be implemented, in which blood will be collected at the same time points. Data will be collected across five visits to the kinesiology laboratory at the University of Ljubljana, Faculty of Sport. Each visit will last approximately 4 hours, except for the first visit where 1RM will be measured.
- Conditions
- BDNF, Irisin, Resistance Exercise, Stroop Test, Lactate, Healthy Young Adult
- Being tested
- Exercise: 80% 1 RM with 2 minutes break, Exercise: 80% 1RM with1 minute break, Exercise: accenuated eccentric exercise, Control
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Exercise: 80% 1 RM with 2 minutes breakOther
Also called resistance exercise
Participant will do resistance exercise at 80% 1RM with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.
Exercise: 80% 1RM with1 minute breakOther
Also called resistance exercise
Participant will do resistance exercise at 80% 1RM with 1 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.
Exercise: accenuated eccentric exerciseOther
Participant will do accenuated eccentric resistance exercise at 80% 1RM in concetric part of movement and 110% 1RM in eccentric part of movement with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.
ControlOther
Timeline of events will be same as in exercise protocol (blood withdrawal and Stroop test), except participants will not do any kind of exercise protocol, instead they will be seating.
| Study group | Role | What they receive |
|---|---|---|
| Intervention: participants going through exercising or control | Experimental | All participants will do 1 control protocol and 3 resistance exercises protocols. Order of protocols will be randomized. Receives: Other: Exercise: 80% 1 RM with 2 minutes break; Other: Exercise: 80% 1RM with1 minute break; Other: Exercise: accenuated eccentric exercise; Other: Control |
What is being measured
The primary outcome is the main question the study is designed to answer.
plasma BDNF
BDNF will be determined in blood plasma with ELISA kit.
Measured: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Irisin
Irisin will be measured in blood plasma.
Measured: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
serum BDNF
BDNF will be determined in blood serum with ELISA kit.
Measured: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Platelet count
Platelet count in blood will be done.
Measured: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
BDNF per platelet
The amount of BDNF per platelet will be calculated.
Measured: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Secondary outcomes (2)
Lactate
Lactate will be measured in blood.
Measured: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Stroop test
Stroop test will be used as an assessment of cognitive functions. The number of read word will be assessed.
Measured: pre, 15 minutes post exercise.
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted23 Sep 2025
- Study start12 May 2025
- Primary completion25 Nov 2025
- Study completionSep 2026 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for BDNFStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Enrolment
- 24 participants (estimated)
Sponsor and investigators
- Lead sponsor
- University of Ljubljana
- Sponsor type
- University, hospital or other
- Responsible party
- Sponsor
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07189130
- Sponsor's protocol ID
- 0120-222/2024-2711-3
- Status last verified
- Dec 2025
- First submitted
- 19 Aug 2025
- Last updated
- 16 Dec 2025
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
Study at a glance
From the registry record, last updated by the sponsor on 16 Dec 2025. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- The Acute Response of Irisin and Brain-derived Neurotrophic Factor in Blood to Resistance Exercise Load
- ClinicalTrials.gov ID
- NCT07189130
- Acronym / sponsor ID
- 0120-222/2024-2711-3
- Conditions
- BDNF, Irisin, Resistance Exercise, Stroop Test, Lactate, Healthy Young Adult
- Study type
- Interventional
- Recruitment status
- Active, not recruiting
- Enrolment
- 24 participants (estimated)
- Lead sponsor
- University of LjubljanaUniversity, hospital or other
- Study start
- 12 May 2025 (actual)
- Primary completion
- 25 Nov 2025 (actual)
- Study completion
- Sep 2026 (estimated)
- First posted
- 23 Sep 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The aim of this study is to investigate different resistance training loads on BNDF and irisin levels in platelets, blood serum and plasma.
Detailed description
In this study, the investigators will examine how three resistance training protocols acutely affect BDNF levels (in serum, plasma, platelet count, and BDNF per platelet) and irisin levels in blood (plasma). The resistance training protocols will differ in intensity, rest intervals between sets and repetition execution. Blood samples will be collected from participants at four time points: before the start of the protocol, 5 minutes after completion of the final set, 1 hour after completion of the final set, and 3 hours after completion of the final set. In addition, blood lactate levels will be monitored at the same time points. Heart rate will also be recorded (immediately before the start of the protocol and 30 seconds after completion of the final set). A control protocol will also be implemented, in which blood will be collected at the same time points. Data will be collected across five visits to the kinesiology laboratory at the University of Ljubljana, Faculty of Sport. Each visit will last approximately 4 hours, except for the first visit where 1RM will be measured.
Study design
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Number of arms
- 1
| Arm | Type | Receives |
|---|---|---|
| Intervention: participants going through exercising or control | Experimental | Other: Exercise: 80% 1 RM with 2 minutes break, Other: Exercise: 80% 1RM with1 minute break, Other: Exercise: accenuated eccentric exercise, Other: ControlAll participants will do 1 control protocol and 3 resistance exercises protocols. Order of protocols will be randomized. |
Who may qualify
- Ages
- 18 to 30 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (2 listed)
- males between 18 and 30 years
- moderate level of physical activity assessed with IPAQ
Key exclusion criteria (6 listed)
- injuries
- cardiovascular diseases
- respiratory diseases
- psychiatric diseases
- metabolic diseases
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Exercise: 80% 1 RM with 2 minutes breakAlso: resistance exerciseParticipant will do resistance exercise at 80% 1RM with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press. | Other | Intervention: participants going through exercising or control |
| Exercise: 80% 1RM with1 minute breakAlso: resistance exerciseParticipant will do resistance exercise at 80% 1RM with 1 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press. | Other | Intervention: participants going through exercising or control |
| Exercise: accenuated eccentric exerciseParticipant will do accenuated eccentric resistance exercise at 80% 1RM in concetric part of movement and 110% 1RM in eccentric part of movement with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press. | Other | Intervention: participants going through exercising or control |
| ControlTimeline of events will be same as in exercise protocol (blood withdrawal and Stroop test), except participants will not do any kind of exercise protocol, instead they will be seating. | Other | Intervention: participants going through exercising or control |
Primary outcome
plasma BDNF
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
BDNF will be determined in blood plasma with ELISA kit.
Irisin
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Irisin will be measured in blood plasma.
serum BDNF
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
BDNF will be determined in blood serum with ELISA kit.
Platelet count
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Platelet count in blood will be done.
BDNF per platelet
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
The amount of BDNF per platelet will be calculated.
2 secondary outcomes
Lactate
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Stroop test
Time frame: pre, 15 minutes post exercise.
Results and safety
This study is still under way. Results are not available in the source record yet.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 16 Dec 2025.
Related trials for BDNF
Not yet recruiting
Virtual Reality Training Dose for Arm Recovery After Stroke: Clinical and Blood Biomarker Responses
Stroke, Upper Extremity Paresis
Enrolling by invitationPhase 2
The Progressive Supranuclear Palsy Clinical Trial Platform - Regimen B: LM11A-31
PSP - Progressive Supranuclear Palsy
RecruitingObservational study
Asprosin and miRNA Expression in Young Women With Metabolic Syndrome
Metabolic Syndrome, Insulin Resistance
Recruiting
Sex Differences in BDNF Study
Exercise, BDNF
RecruitingObservational study
Association Between Circulating BDNF Levels and Atrial Cardiomyopathy in Patients Undergoing Ablation for Persistent Atrial Fibrillation
Atrial Fibrillation (AF), Cardiac Arrhythmia
Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Exercise: 80% 1 RM with 2 minutes break, Exercise: 80% 1RM with1 minute break and Exercise: accenuated eccentric exercise in people with BDNF and Irisin, run by University of Ljubljana at 1 site in Slovenia.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 2 inclusion and 6 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: under way, not looking for participants, as of the sponsor's last update on 16 Dec 2025. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Slovenia. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
This study is still under way. Results are not available in the source record yet.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
plasma BDNF (time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.). The study lists 5 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 16 Dec 2025.