Recovery MethodsNCT07801924
Evaluation of the Effectiveness of Oral Ozonized Oil Supplementation in Elite Athletes
A study testing Ozonized Oil in people with Recovery Methods and Strength Endurance, run by Universita degli Studi di Genova at 2 sites in Italy.
Trial summary
Ozonized oil has anti-inflammatory and antioxidant properties, but its effects on athletic performance remain unclear. This study evaluated whether daily ozonized oil supplementation, combined with training, influences aerobic capacity, anaerobic power, maximal strength, and muscular endurance in elite rugby players… Read more about this study
- Who is it for?
- Ages 18 years and older, men onlyHealthy volunteers can take part
- Conditions the study is for
- Recovery Methods, Strength Endurance, Muscular
- What is being tested?
- Ozonized Oil
- Type of treatment
- Pill or oral medicine
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 33 participants
- Where
- 2 sitesItaly
- Study dates
- 9 Sep 2024to 18 Apr 2025
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 years and older
- Men only
- Healthy volunteers accepted
- Adult, Older adult
Who may be able to join
None listed.
Who cannot join
- NA
Trial locations and contacts
2 sites in 1 country.
Italy2 sites
Stadio del Rugby
Avezzano, AQ, 67051
University of Genova
Genova, Genova, 16132
About this study
Official title: Evaluation of the Effects of Ozonated Oil Administration on Athletic Performance and Injury Prevention in Elite Athletes.
Ozonized oil has anti-inflammatory and antioxidant properties, but its effects on athletic performance remain unclear. This study evaluated whether daily ozonized oil supplementation, combined with training, influences aerobic capacity, anaerobic power, maximal strength, and muscular endurance in elite rugby players over six months
- Conditions
- Recovery Methods, Strength Endurance, Muscular
- Being tested
- Ozonized Oil
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Ozonized OilDietary supplement
, the present study aimed to investigate the potential effects of daily oral supplementation with ozonized oil (O3ZONE Sport®) (produced for O3 ZONE LIMITED, Acquaviva, RSM) on physical performance outcomes - aerobic capacity, maximal strength, muscular endurance, and functional fitness - in elite rugby players over a six-month training period.
| Study group | Role | What they receive |
|---|---|---|
| Intervention group (IG): received daily ozonized oil supplementation | Experimental | The IG received the supplement of ozonized oil capsules (O3ZONE Sport ®). Dosage was standardized and adjusted according to body:
* ≥100 kg: 6 capsules/day (3 morning, 3 evening)
* <100 kg: 4 capsules/day (2 morning, 2 evening) Adherence to the protocol was monitored weekly through self-report and supervision by team medical staff.
Both groups followed identical training protocols; the IG additionally received daily ozonized oil. Performance was assessed at baseline, 3 months, and 6 months using the Bronco test (field endurance), Bruce and Léger tests (estimated VO2max), the Running-Based Anaerobic Sprint Test (RAST), and tests of quadriceps and bench-press 1-repetition maximum (1RM) and endurance at 50% 1RM. Outcomes were expressed as percentage change from baseline Receives: Dietary Supplement: Ozonized Oil |
| • Control group (CG): n = 15, which continued standard training without supplementation | No intervention | Not described |
What is being measured
The primary outcome is the main question the study is designed to answer.
The primary objective was to investigate the effects of ozonated oil administration on athletic performance and injury prevention in a group of 21 rugby athletes, divided into two groups: Experimental group (n=13) vs Control group (n=8)
Bruce test: which measures a subject's maximal oxygen consumption (VO2max). VO2max represents the maximum aerobic power, that is, the maximum amount of energy that can be synthesized by the aerobic mechanism in a unit of time.
Measured: 1 year
Secondary outcomes (2)
The primary objective was to investigate the effects of ozonated oil administration on athletic performance and injury prevention in a group of 21 rugby athletes, divided into two groups: Experimental group (n=13) vs Control group (n=8)
Bronco test: is a 1,200 meter shuttle run designed to measure maximum aerobic speed (MAS) and endurance, primarily used in rugby. The test requires athletes to complete five sets of 20 meter, 40 meter, and 60meter shuttle runs, totalling 1,200 meters as fast as possible
Measured: 1 year
The primary objective was to investigate the effects of ozonated oil administration on athletic performance and injury prevention in a group of 21 rugby athletes, divided into two groups: Experimental group (n=13) vs Control group (n=8)
Leger Test:or multi-stage fitness test, requires running back and forth between two markers placed exactly 20 meters (65.6 feet) apart. The pace is set by audio beeps, with speeds increasing every minute (level), typically starting at 8.5 km/h and increasing by 0.5 km/h each level
Measured: 1 year
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted3 Sep 2026
- Study start9 Sep 2024
- Primary completion17 Apr 2025
- Study completion18 Apr 2025
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Recovery MethodsStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Supportive care
- Allocation
- Randomised
- Intervention model
- Single group
- Masking
- Single blind
- Who is masked
- Participant
- Enrolment
- 33 participants (actual)
Sponsor and investigators
- Lead sponsor
- Universita degli Studi di Genova
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Izzotti, Alberto, Universita degli Studi di Genova
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07801924
- Sponsor's protocol ID
- C1LN001
- Other IDs
- 100008-2024-AIFFRA
- Status last verified
- Apr 2026
- First submitted
- 16 Apr 2026
- Last updated
- 3 Sep 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- Undecided
Study at a glance
From the registry record, last updated by the sponsor on 3 Sep 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Evaluation of the Effects of Ozonated Oil Administration on Athletic Performance and Injury Prevention in Elite Athletes.
- ClinicalTrials.gov ID
- NCT07801924
- Acronym / sponsor ID
- C1LN001
- Conditions
- Recovery Methods, Strength Endurance, Muscular
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 33 participants (actual)
- Lead sponsor
- Universita degli Studi di GenovaUniversity, hospital or other
- Study start
- 9 Sep 2024 (actual)
- Primary completion
- 17 Apr 2025 (actual)
- Study completion
- 18 Apr 2025 (actual)
- First posted
- 3 Sep 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Ozonized oil has anti-inflammatory and antioxidant properties, but its effects on athletic performance remain unclear. This study evaluated whether daily ozonized oil supplementation, combined with training, influences aerobic capacity, anaerobic power, maximal strength, and muscular endurance in elite rugby players over six months
Study design
- Primary purpose
- Supportive care
- Allocation
- Randomised
- Intervention model
- Single group
- Masking
- Single blind (participant)
- Number of arms
- 2
| Arm | Type | Receives |
|---|---|---|
| Intervention group (IG): received daily ozonized oil supplementation | Experimental | Dietary Supplement: Ozonized OilThe IG received the supplement of ozonized oil capsules (O3ZONE Sport ®). Dosage was standardized and adjusted according to body: * ≥100 kg: 6 capsules/day (3 morning, 3 evening) * <100 kg: 4 capsules/day (2 morning, 2 evening) Adherence to the protocol was monitored weekly through self-report and supervision by team medical staff. Both groups followed identical training protocols; the IG additionally received daily ozonized oil. Performance was assessed at baseline, 3 months, and 6 months using the Bronco test (field endurance), Bruce and Léger tests (estimated VO2max), the Running-Based Anaerobic Sprint Test (RAST), and tests of quadriceps and bench-press 1-repetition maximum (1RM) and endurance at 50% 1RM. Outcomes were expressed as percentage change from baseline |
| • Control group (CG): n = 15, which continued standard training without supplementation | No intervention | – |
Who may qualify
- Ages
- 18 years and older
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult, Older adult
Key inclusion criteria (0 listed)
Key exclusion criteria (1 listed)
- NA
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Ozonized Oil, the present study aimed to investigate the potential effects of daily oral supplementation with ozonized oil (O3ZONE Sport®) (produced for O3 ZONE LIMITED, Acquaviva, RSM) on physical performance outcomes - aerobic capacity, maximal strength, muscular endurance, and functional fitness - in elite rugby players over a six-month training period. | Dietary supplement | Intervention group (IG): received daily ozonized oil supplementation |
Primary outcome
The primary objective was to investigate the effects of ozonated oil administration on athletic performance and injury prevention in a group of 21 rugby athletes, divided into two groups: Experimental group (n=13) vs Control group (n=8)
Time frame: 1 year
Bruce test: which measures a subject's maximal oxygen consumption (VO2max). VO2max represents the maximum aerobic power, that is, the maximum amount of energy that can be synthesized by the aerobic mechanism in a unit of time.
2 secondary outcomes
The primary objective was to investigate the effects of ozonated oil administration on athletic performance and injury prevention in a group of 21 rugby athletes, divided into two groups: Experimental group (n=13) vs Control group (n=8)
Time frame: 1 year
The primary objective was to investigate the effects of ozonated oil administration on athletic performance and injury prevention in a group of 21 rugby athletes, divided into two groups: Experimental group (n=13) vs Control group (n=8)
Time frame: 1 year
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 3 Sep 2026.
- Individual participant dataSharing planned: Undecided
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
2 sites in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Ozonized Oil in people with Recovery Methods and Strength Endurance, run by Universita degli Studi di Genova at 2 sites in Italy.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 0 inclusion and 1 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 3 Sep 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 2 sites in Italy. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
The primary objective was to investigate the effects of ozonated oil administration on athletic performance and injury prevention in a group of 21 rugby athletes, divided into two groups: Experimental group (n=13) vs Control group (n=8) (time frame: 1 year). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 3 Sep 2026.