Exercise ImmunologyWBHIIT-IMMNCT07618455
Effects of Whole-Body HIIT and Moderate Continuous Exercise on Immune Function
A study testing Whole-Body High-Intensity Interval Training (WB-HIIT) and Moderate-Intensity Continuous Training (MICT) in people with Exercise Immunology and Exercise Physiology, run by Bitlis Eren University at 1 site in Turkey (Türkiye).
Trial summary
This randomized controlled trial aimed to investigate the effects of a 12-week Whole-Body High-Intensity Interval Training (WB-HIIT) program compared with Moderate-Intensity Continuous Training (MICT) on immune function in recreationally active young adults. Read more about this study
- Who is it for?
- Ages 18 years and older, men onlyHealthy volunteers can take part
- Conditions the study is for
- Exercise Immunology, Exercise Physiology
- What is being tested?
- Whole-Body High-Intensity Interval Training (WB-HIIT) and Moderate-Intensity Continuous Training (MICT)
- Type of treatment
- Lifestyle or therapy programme
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 20 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 30 Jun 2020to 30 Aug 2020
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 years and older
- Men only
- Healthy volunteers accepted
- Adult, Older adult
Who may be able to join
- Healthy male adults Aged 18-25 years Not engaged in regular structured exercise training (>3 sessions/week) VO₂max within normal healthy range Willingness to participate and provide informed consent No acute or chronic inflammatory, metabolic, or cardiovascular disease
Who cannot join
- Female participants (not included in study design) Smoking or alcohol abuse Use of anti-inflammatory or immunomodulatory drugs Participation in regular high-intensity training programs Musculoskeletal limitations preventing exercise testing or training Acute infection within last 2 weeks Chronic metabolic, cardiovascular, or autoimmune diseases
Trial locations and contacts
1 site in 1 country.
About this study
Official title: Effects of Whole-Body High-Intensity Interval Training and Moderate-Intensity Continuous Exercise on Immune Function in Recreationally Active Men
This randomized controlled trial aimed to investigate the effects of a 12-week Whole-Body High-Intensity Interval Training (WB-HIIT) program compared with Moderate-Intensity Continuous Training (MICT) on immune function in recreationally active young adults. Participants were randomly assigned to either WB-HIIT or MICT groups. Immune-related biomarkers and performance outcomes were assessed before and after the intervention. The study sought to determine whether different exercise modalities produce distinct adaptations in immune function.
Read the detailed description
This study was designed as a two-arm, parallel-group, randomized controlled trial. A total of 20 recreationally active male participants were randomly assigned to either the Whole Body High-Intensity Interval Training (WB-HIIT) group or the Moderate-Intensity Continuous Training (MICT) group. The intervention lasted 12 weeks and involved supervised training sessions multiple times a week.
The WB-HIIT protocol consisted of repeated high-intensity whole-body exercises performed at near-maximum effort with intermittent rest periods, while the MICT group performed moderate-intensity continuous aerobic exercise. Immune function was assessed through selected hematological and biochemical markers collected at baseline and post-intervention. In addition, physical performance variables were evaluated to examine secondary adaptations to different exercise modalities. The primary aim of the study was to compare the effects of WB-HIIT and MICT on immune system responses, while secondary outcomes included changes in exercise performance. This study provides insights into how different training intensities affect immune regulation in individuals who are active in their leisure time.
- Conditions
- Exercise Immunology, Exercise Physiology
- Being tested
- Whole-Body High-Intensity Interval Training (WB-HIIT), Moderate-Intensity Continuous Training (MICT)
- Keywords
- High-intensity interval training, moderate-intensity continuous exercise, immune system, ACTH, cortisol, exercise immunology, IL-6
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Whole-Body High-Intensity Interval Training (WB-HIIT)Behavioural
Whole-body high-intensity interval training protocol performed under supervision.
Moderate-Intensity Continuous Training (MICT)Behavioural
Moderate-intensity continuous training protocol performed under supervision.
| Study group | Role | What they receive |
|---|---|---|
| Whole-Body HIIT | Experimental | Participants will perform Whole-Body High-Intensity Interval Training (WB-HIIT) protocol. Receives: Behavioral: Whole-Body High-Intensity Interval Training (WB-HIIT) |
| MICT | Active comparator | Participants will perform Moderate-Intensity Continuous Training (MICT) protocol. Receives: Behavioral: Moderate-Intensity Continuous Training (MICT) |
What is being measured
The primary outcome is the main question the study is designed to answer.
Peripheral immune cell counts
Changes in peripheral blood leukocyte, lymphocyte subsets, neutrophil, and monocyte counts from baseline to post-intervention (8 weeks).
Measured: Baseline and 8 weeks (post-intervention)
Secondary outcomes (4)
Inflammatory cytokine levels
Changes in serum IL-6, TNF-α, and CRP levels from baseline to post-intervention (8 weeks).
Measured: Baseline and 8 weeks (post-intervention)
Immunoglobulin levels
Changes in serum immunoglobulin (IgA, IgG, IgM) concentrations from baseline to post-intervention (8 weeks).
Measured: Baseline and 8 weeks (post-intervention)
Cortisol levels
Changes in serum cortisol levels as a marker of exercise-induced stress response from baseline to post-intervention (8 weeks).
Measured: Baseline and 8 weeks (post-intervention)
Maximal oxygen uptake (VO₂max)
Changes in VO₂max measured by cardiopulmonary exercise testing from baseline to post-intervention.
Measured: Baseline and 8 weeks (post-intervention)
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted1 Jun 2026
- Study start30 Jun 2020
- Primary completion30 Aug 2020
- Study completion30 Aug 2020
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Exercise ImmunologyStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Model description
- Participants will be randomly assigned to either Whole-Body High-Intensity Interval Training (WB-HIIT) or Moderate-Intensity Continuous Training (MICT) groups.
- Masking
- None (open label)
- Enrolment
- 20 participants (actual)
Sponsor and investigators
- Lead sponsor
- Bitlis Eren University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Melih Calişir, Bitlis Eren University
- Investigators
- Melih Çalışır, PhD, Principal investigator, Bitlis Eren Üniversitesi University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07618455
- Sponsor's protocol ID
- 2020-189
- Acronym
- WBHIIT-IMM
- Status last verified
- May 2026
- First submitted
- 16 May 2026
- Last updated
- 1 Jun 2026
- Results posted
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No: Individual participant data will not be shared due to participant privacy and confidentiality concerns.
Study at a glance
From the registry record, last updated by the sponsor on 1 Jun 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Effects of Whole-Body High-Intensity Interval Training and Moderate-Intensity Continuous Exercise on Immune Function in Recreationally Active Men
- ClinicalTrials.gov ID
- NCT07618455
- Acronym / sponsor ID
- WBHIIT-IMM / 2020-189
- Conditions
- Exercise Immunology, Exercise Physiology
- Keywords
- High-intensity interval training, moderate-intensity continuous exercise, immune system, ACTH, cortisol, exercise immunology, IL-6
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 20 participants (actual)
- Lead sponsor
- Bitlis Eren UniversityUniversity, hospital or other
- Study start
- 30 Jun 2020 (actual)
- Primary completion
- 30 Aug 2020 (actual)
- Study completion
- 30 Aug 2020 (actual)
- First posted
- 1 Jun 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
This randomized controlled trial aimed to investigate the effects of a 12-week Whole-Body High-Intensity Interval Training (WB-HIIT) program compared with Moderate-Intensity Continuous Training (MICT) on immune function in recreationally active young adults. Participants were randomly assigned to either WB-HIIT or MICT groups. Immune-related biomarkers and performance outcomes were assessed before and after the intervention. The study sought to determine whether different exercise modalities produce distinct adaptations in immune function.
Detailed description
This study was designed as a two-arm, parallel-group, randomized controlled trial. A total of 20 recreationally active male participants were randomly assigned to either the Whole Body High-Intensity Interval Training (WB-HIIT) group or the Moderate-Intensity Continuous Training (MICT) group. The intervention lasted 12 weeks and involved supervised training sessions multiple times a week.
The WB-HIIT protocol consisted of repeated high-intensity whole-body exercises performed at near-maximum effort with intermittent rest periods, while the MICT group performed moderate-intensity continuous aerobic exercise. Immune function was assessed through selected hematological and biochemical markers collected at baseline and post-intervention. In addition, physical performance variables were evaluated to examine secondary adaptations to different exercise modalities. The primary aim of the study was to compare the effects of WB-HIIT and MICT on immune system responses, while secondary outcomes included changes in exercise performance. This study provides insights into how different training intensities affect immune regulation in individuals who are active in their leisure time.
Study design
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- None (open label)
- Number of arms
- 2
- Model description
- Participants will be randomly assigned to either Whole-Body High-Intensity Interval Training (WB-HIIT) or Moderate-Intensity Continuous Training (MICT) groups.
| Arm | Type | Receives |
|---|---|---|
| Whole-Body HIIT | Experimental | Behavioral: Whole-Body High-Intensity Interval Training (WB-HIIT)Participants will perform Whole-Body High-Intensity Interval Training (WB-HIIT) protocol. |
| MICT | Active comparator | Behavioral: Moderate-Intensity Continuous Training (MICT)Participants will perform Moderate-Intensity Continuous Training (MICT) protocol. |
Who may qualify
- Ages
- 18 years and older
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult, Older adult
Key inclusion criteria (1 listed)
- Healthy male adults Aged 18-25 years Not engaged in regular structured exercise training (>3 sessions/week) VO₂max within normal healthy range Willingness to participate and provide informed consent No acute or chronic inflammatory, metabolic, or cardiovascular disease
Key exclusion criteria (1 listed)
- Female participants (not included in study design) Smoking or alcohol abuse Use of anti-inflammatory or immunomodulatory drugs Participation in regular high-intensity training programs Musculoskeletal limitations preventing exercise testing or training Acute infection within last 2 weeks Chronic metabolic, cardiovascular, or autoimmune diseases
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Whole-Body High-Intensity Interval Training (WB-HIIT)Whole-body high-intensity interval training protocol performed under supervision. | Behavioural | Whole-Body HIIT |
| Moderate-Intensity Continuous Training (MICT)Moderate-intensity continuous training protocol performed under supervision. | Behavioural | MICT |
Primary outcome
Peripheral immune cell counts
Time frame: Baseline and 8 weeks (post-intervention)
Changes in peripheral blood leukocyte, lymphocyte subsets, neutrophil, and monocyte counts from baseline to post-intervention (8 weeks).
4 secondary outcomes
Inflammatory cytokine levels
Time frame: Baseline and 8 weeks (post-intervention)
Immunoglobulin levels
Time frame: Baseline and 8 weeks (post-intervention)
Cortisol levels
Time frame: Baseline and 8 weeks (post-intervention)
Maximal oxygen uptake (VO₂max)
Time frame: Baseline and 8 weeks (post-intervention)
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 1 Jun 2026.
- Individual participant dataSharing planned: No. Individual participant data will not be shared due to participant privacy and confidentiality concerns.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
Melih Çalışır, PhD principal investigator
Bitlis Eren Üniversitesi University
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Whole-Body High-Intensity Interval Training (WB-HIIT) and Moderate-Intensity Continuous Training (MICT) in people with Exercise Immunology and Exercise Physiology, run by Bitlis Eren University at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 1 inclusion and 1 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 1 Jun 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Peripheral immune cell counts (time frame: Baseline and 8 weeks (post-intervention)). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 1 Jun 2026.