Chronic Apical PeriodontitisNCT07529964

MMP-2/3 and Periapical Healing Outcomes

An observational study of Chronic Apical Periodontitis and Periapical Healing, run by TC Erciyes University.

Not yet recruiting

Opening soon. The study is approved but enrolment has not started yet. Sites may already take enquiries.

Official record

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Trial summary

Who is it for?: Ages 18 to 65 years
Conditions the study is for: Chronic Apical Periodontitis, Periapical Healing
What is being tested?: Nonsurgical Root Canal Treatment
Type of treatment: Surgery or procedure
How many people: 50 participants
Where: No sites listed yet
Study dates: 15 May 2026to 25 Jun 2027
Phase: Observational

Key requirements

Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.

Ages 18 to 65 years
All sexes
No healthy volunteers
Adult, Older adult

Study population: The study population will consist of adult patients aged 18-65 years diagnosed with chronic apical periodontitis in single-rooted teeth and requiring primary nonsurgical root canal treatment. All participants will be classified as ASA I or II and will be recruited from patients referred to the Department of Endodontics. Only individuals meeting the predefined inclusion and exclusion criteria will be enrolled. Participants will undergo standardized treatment and follow-up under controlled clinical conditions.

Who may be able to join

Single-rooted teeth
PAI score ≥ 3
Fully developed root apices
Periodontal probing depth less than 5 mm
Indication for primary nonsurgical root canal treatment

Who cannot join

Previous endodontic treatment in the selected tooth
Diagnosis of symptomatic apical abscess
Presence of root fracture or internal/external resorption
Use of systemic antibiotics within the previous 4 weeks
Analgesic intake within 48 hours before treatment
Ongoing immunosuppressive therapy
Pregnancy or breastfeeding

About this study

Official title: Association of MMP-2 and MMP-3 Polymorphisms With 12-Month Radiographic Healing After Nonsurgical Root Canal Treatment in Chronic Apical Periodontitis: A Prospective Clinical Study

This prospective randomized clinical study will investigate the association between MMP-2 and MMP-3 gene polymorphisms and radiographic healing outcomes following nonsurgical root canal treatment in patients with chronic apical periodontitis. A total of 50 patients will undergo standardized endodontic treatment and will be followed for 12 months. Genotyping will be performed using baseline DNA samples, and periapical healing will be assessed using the Periapical Index (PAI). The relationship between genetic variations and treatment outcomes will be analyzed to determine whether host-related genetic factors influence healing patterns after root canal therapy.

Conditions: Chronic Apical Periodontitis, Periapical Healing
Being tested: Nonsurgical Root Canal Treatment

What participants receive

What participants receive. In a randomised study each person is assigned to one group by chance.

Nonsurgical Root Canal TreatmentProcedure or surgery
All participants will undergo standardized nonsurgical root canal treatment performed under a uniform clinical protocol. After local anesthesia and rubber dam isolation, working length will be determined electronically and radiographically. Root canal preparation will be completed with rotary instrumentation up to size 30/.06, accompanied by sodium hypochlorite irrigation, EDTA, and ultrasonic activation. Canals will then be obturated in the same visit using gutta-percha and epoxy resin-based sealer with the warm vertical condensation technique. Periapical healing outcomes at 12 months will be evaluated in relation to MMP-2 and MMP-3 genetic polymorphisms.

Study groups in NCT07529964
Study group	Role	What they receive
Standardized Root Canal Treatment		Participants will undergo standardized nonsurgical root canal treatment using a uniform clinical protocol. Radiographic healing outcomes will be evaluated at 12 months, and results will be analyzed in relation to MMP-2 and MMP-3 gene polymorphisms. Receives: Procedure: Nonsurgical Root Canal Treatment

What is being measured

The primary outcome is the main question the study is designed to answer.

Radiographic Periapical Healing
The primary outcome will be radiographic healing assessed using the Periapical Index (PAI) scoring system on standardized periapical radiographs.
Measured: 12 months after treatment

Secondary outcomes (1)

Association Between MMP Genotypes and Healing Outcomes
The association between MMP-2 (rs243865) and MMP-3 (rs3025058) genotypes and radiographic healing outcomes will be evaluated. Genotype and allele distributions will be compared between healed and non-healed groups to determine the potential influence of genetic polymorphisms on periapical healing following root canal treatment.
Measured: Baseline (genotyping) and 12 months after treatment

Timeline

From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.

15 May 202625 Jun 2027

First posted14 Apr 2026
Study start15 May 2026 (estimated)
Primary completion25 May 2027 (estimated)
Study completion25 Jun 2027 (estimated)

Resources

Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
Other trials for Chronic Apical PeriodontitisStudies of the same condition, open and under way.
What the trial phases meanHow a treatment moves from first tests in people to approval.

More details

For doctors and researchers: how the study is designed, who runs it, and its registry IDs.

Study design

Study type: Observational
Observational model: Cohort
Time perspective: Prospective
Enrolment: 50 participants (estimated)

IDs, dates and publications

ClinicalTrials.gov ID: NCT07529964
Sponsor's protocol ID: 3
Status last verified: Apr 2026
First submitted: 8 Apr 2026
Last updated: 14 Apr 2026
Results posted: No
Data monitoring committee: No
FDA-regulated drug: No
FDA-regulated device: No
Individual data sharing: No

Interested in taking part?

The sponsor has not listed a contact. The official record may have more details.

Talk to your doctor first and take this page with you. Only the study team can confirm whether you can join.

Questions to ask the study team

What is the study trying to find out?
What would I need to do, and how often would I visit?
Could I receive a placebo instead of the treatment?
What are the possible risks and benefits for me?
Will it cost me anything? Are travel or time paid for?
Can I keep seeing my own doctor during the study?
What happens when the study ends?

Official record

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