Evaluation of Postoperative Pain and Antimicrobial EfficacyNRSNCT07852260
Evaluation of Postoperative Pain and the Antimicrobial Effect of Super Oxidized Water (Microcyn) and Chlorhexidine When Compared With Sodium Hypochlorite in Teeth With Asymptomatic Necrotic Pulps. A Randomized Clinical Trial
A study testing super-oxidized water (Microcyn) irrigating solution, 2% chlorhexidine irrigating solution and 2.5% sodium hypochlorite in people with Evaluation of Postoperative Pain and Antimicrobial Efficacy, run by Cairo University at 1 site in Egypt.
Trial summary
The objective of this study is to evaluate the postoperative pain and antimicrobial efficacy when using super-oxidized water (Microcyn) and 2% Chlorhexidine as irrigating solutions in comparison to 2.5% Sodium hypochlorite in teeth with asymptomatic necrotic pulps. Read more about this study
- Who is it for?
- Ages 18 to 50 years
- Conditions the study is for
- Evaluation of Postoperative Pain and Antimicrobial Efficacy
- What is being tested?
- super-oxidized water (Microcyn) irrigating solution, 2% chlorhexidine irrigating solution and 2.5% sodium hypochlorite
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 75 participants
- Where
- 1 siteEgypt
- Study dates
- 2 Nov 2026to 2 Nov 2027
- Phase
- Interventional
- Are there results?
- Not yet: the study is under way
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 50 years
- All sexes
- No healthy volunteers
- Adult
Who may be able to join
- Patients in good physical health; American Society of Anesthesiologists (ASA) class I or II.
- Patients whose age ranges between 18 and 50 years.
- Male and female patients.
- Patients having a single canal-tooth with asymptomatic necrotic pulp.
- Patients who can understand the number rating scale.
- Patients who accept to participate in the trial and can sign the informed consent
Who cannot join
- Pregnant females.
- Patients with intraoral or extraoral swelling.
- Patients whose tooth is non-restorable.
- Teeth with open apices.
- Teeth with radiographic evidence of root resorption.
Trial locations and contacts
1 site in 1 country. Contact the study team or a site near you to ask about taking part.
Egypt1 site
faculty of Dentistry Cairo university
Cairo, Cairo Governorate, 11553
Paunceh S Soliman, PHD candidate, 01026999750, paunceh.soliman@dentistry.cu.edu.eg
About this study
Official title: Evaluation of Postoperative Pain and the Antimicrobial Effect of Super Oxidized Water (Microcyn) and Chlorhexidine When Compared With Sodium Hypochlorite in Teeth With Asymptomatic Necrotic Pulps A Randomized Clinical Trial
The objective of this study is to evaluate the postoperative pain and antimicrobial efficacy when using super-oxidized water (Microcyn) and 2% Chlorhexidine as irrigating solutions in comparison to 2.5% Sodium hypochlorite in teeth with asymptomatic necrotic pulps.
Read the detailed description
The objective of this study is to evaluate the postoperative pain and antimicrobial efficacy when using super-oxidized water (Microcyn) and 2% Chlorhexidine as irrigating solutions in comparison to 2.5% Sodium hypochlorite in teeth with asymptomatic necrotic pulps.
- Being tested
- super-oxidized water (Microcyn) irrigating solution, 2% chlorhexidine irrigating solution, 2.5% sodium hypochlorite
- Keywords
- Postoperative pain, NRS, Bacterial load reduction
- Medical subject headings
- Pain, Postoperative
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
super-oxidized water (Microcyn) irrigating solutionOther
The difference in concentration of chlorine between NaOCl and super oxidized water such that the concentration of chlorine ions in NaOCl is much higher than that in super-oxidized water. However, considering the 90% reduction in biofilm thickness and 98% reduction in colony count of microorganisms by super-oxidized water as demonstrated
2% chlorhexidine irrigating solutionOther
Chlorhexidine (CHX) has been indicated as an alternative irrigation agent in the preparation of the root canal system, due to its broad-spectrum antimicrobial capacity, dentin tubule disinfection, dentin adsorption, high substantivity, and low toxicity compared to NaOCl. The inability to dissolve organic tissues of the CHX provides controversial in the use of this irrigant, and due to this limitation, some authors do not indicate it as the main irrigation solution in endodontic treatment despite its utility as a final irrigation solution.
2.5% sodium hypochloriteOther
Sodium hypochlorite (NaOCl) is the most common and has been widely used in endodontics for more than 70 years because of its ability to act as a solvent for organic matter and its efficient antimicrobial action, however, at high concentrations, it is likely to irritate periradicular tissues.
| Study group | Role | What they receive |
|---|---|---|
| Group A | Active comparator | Each canal will receive 2ml of super-oxidized water (Microcyn) irrigating solution between each instrument size and the subsequent one. Receives: Other: super-oxidized water (Microcyn) irrigating solution |
| Group B | Active comparator | Each canal will receive 2ml of 2% chlorhexidine irrigating solution between each instrument size and the subsequent one. Receives: Other: 2% chlorhexidine irrigating solution |
| Group C | Active comparator | Each canal will receive 2ml of 2.5 % sodium hypochlorite irrigating solution between each instrument size and the subsequent one. Receives: Other: 2.5% sodium hypochlorite |
What is being measured
The primary outcome is the main question the study is designed to answer.
Postoperative pain intensity at 6 hours post-instrumentation
A pain chart using numerical rating scale (NRS) will then be used to record the patients' pain level.
The NRS (0-10 scale) consists of a line anchored by two extremes "No pain" and "the worst pain".
Patients will be asked to choose the mark that represented their level of pain from 0 to 10. Pain level will be assigned as follow: 0: no pain, 1-3: mild pain, 4-6: moderate pain, 7-10: severe pain.
Measured: 6 hours post-instrumentation
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted1 Oct 2026
- Study start2 Nov 2026 (estimated)
- Primary completion2 Nov 2027 (estimated)
- Study completion2 Nov 2027 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Evaluation of Postoperative Pain and Antimicrobial EfficacyStudies of the same condition, open and under way.
- More trials run by Cairo UniversityThe sponsor's other registered studies.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Factorial
- Model description
- Group A: Each canal will receive 2ml of super-oxidized water (Microcyn) irrigating solution between each instrument size and the subsequent one. Group B: Each canal will receive 2ml of 2% chlorhexidine irrigating solution between each instrument size and the subsequent one. Group C: Each canal will receive 2ml of 2.5 % sodium hypochlorite irrigating solution between each instrument size and the subsequent one.
- Masking
- Quadruple blind
- Who is masked
- Participant, Care provider, Investigator, Outcomes assessor
- Enrolment
- 75 participants (estimated)
Sponsor and investigators
- Lead sponsor
- Cairo University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Paunceh Samy Hassan Soliman, Cairo University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07852260
- Sponsor's protocol ID
- Paunceh samy
- Acronym
- NRS
- Status last verified
- Sep 2026
- First submitted
- 25 Sep 2026
- Last updated
- 1 Oct 2026
- Results posted
- No
- Data monitoring committee
- Yes
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No: There is no plan to share individual participant data (IPD) with other researchers due to participant confidentiality and privacy considerations.
Study at a glance
From the registry record, last updated by the sponsor on 1 Oct 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Evaluation of Postoperative Pain and the Antimicrobial Effect of Super Oxidized Water (Microcyn) and Chlorhexidine When Compared With Sodium Hypochlorite in Teeth With Asymptomatic Necrotic Pulps A Randomized Clinical Trial
- ClinicalTrials.gov ID
- NCT07852260
- Acronym / sponsor ID
- NRS / Paunceh samy
- Keywords
- Postoperative pain, NRS, Bacterial load reduction
- Study type
- Interventional
- Recruitment status
- Not yet recruiting
- Enrolment
- 75 participants (estimated)
- Lead sponsor
- Cairo UniversityUniversity, hospital or other
- Study start
- 2 Nov 2026 (estimated)
- Primary completion
- 2 Nov 2027 (estimated)
- Study completion
- 2 Nov 2027 (estimated)
- First posted
- 1 Oct 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The objective of this study is to evaluate the postoperative pain and antimicrobial efficacy when using super-oxidized water (Microcyn) and 2% Chlorhexidine as irrigating solutions in comparison to 2.5% Sodium hypochlorite in teeth with asymptomatic necrotic pulps.
Detailed description
The objective of this study is to evaluate the postoperative pain and antimicrobial efficacy when using super-oxidized water (Microcyn) and 2% Chlorhexidine as irrigating solutions in comparison to 2.5% Sodium hypochlorite in teeth with asymptomatic necrotic pulps.
Study design
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Factorial
- Masking
- Quadruple blind (participant, care provider, investigator, outcomes assessor)
- Number of arms
- 3
- Model description
- Group A: Each canal will receive 2ml of super-oxidized water (Microcyn) irrigating solution between each instrument size and the subsequent one. Group B: Each canal will receive 2ml of 2% chlorhexidine irrigating solution between each instrument size and the subsequent one. Group C: Each canal will receive 2ml of 2.5 % sodium hypochlorite irrigating solution between each instrument size and the subsequent one.
| Arm | Type | Receives |
|---|---|---|
| Group A | Active comparator | Other: super-oxidized water (Microcyn) irrigating solutionEach canal will receive 2ml of super-oxidized water (Microcyn) irrigating solution between each instrument size and the subsequent one. |
| Group B | Active comparator | Other: 2% chlorhexidine irrigating solutionEach canal will receive 2ml of 2% chlorhexidine irrigating solution between each instrument size and the subsequent one. |
| Group C | Active comparator | Other: 2.5% sodium hypochloriteEach canal will receive 2ml of 2.5 % sodium hypochlorite irrigating solution between each instrument size and the subsequent one. |
Who may qualify
- Ages
- 18 to 50 years
- Sex
- All
- Healthy volunteers
- Not accepted
- Age groups
- Adult
Key inclusion criteria (6 listed)
- Patients in good physical health; American Society of Anesthesiologists (ASA) class I or II.
- Patients whose age ranges between 18 and 50 years.
- Male and female patients.
- Patients having a single canal-tooth with asymptomatic necrotic pulp.
- Patients who can understand the number rating scale.
Key exclusion criteria (5 listed)
- Pregnant females.
- Patients with intraoral or extraoral swelling.
- Patients whose tooth is non-restorable.
- Teeth with open apices.
- Teeth with radiographic evidence of root resorption.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| super-oxidized water (Microcyn) irrigating solutionThe difference in concentration of chlorine between NaOCl and super oxidized water such that the concentration of chlorine ions in NaOCl is much higher than that in super-oxidized water. However, considering the 90% reduction in biofilm thickness and 98% reduction in colony count of microorganisms by super-oxidized water as demonstrated | Other | Group A |
| 2% chlorhexidine irrigating solutionChlorhexidine (CHX) has been indicated as an alternative irrigation agent in the preparation of the root canal system, due to its broad-spectrum antimicrobial capacity, dentin tubule disinfection, dentin adsorption, high substantivity, and low toxicity compared to NaOCl. The inability to dissolve organic tissues of the CHX provides controversial in the use of this irrigant, and due to this limitation, some authors do not indicate it as the main irrigation solution in endodontic treatment despite its utility as a final irrigation solution. | Other | Group B |
| 2.5% sodium hypochloriteSodium hypochlorite (NaOCl) is the most common and has been widely used in endodontics for more than 70 years because of its ability to act as a solvent for organic matter and its efficient antimicrobial action, however, at high concentrations, it is likely to irritate periradicular tissues. | Other | Group C |
Primary outcome
Postoperative pain intensity at 6 hours post-instrumentation
Time frame: 6 hours post-instrumentation
A pain chart using numerical rating scale (NRS) will then be used to record the patients' pain level. The NRS (0-10 scale) consists of a line anchored by two extremes "No pain" and "the worst pain". Patients will be asked to choose the mark that represented their level of pain from 0 to 10. Pain level will be assigned as follow: 0: no pain, 1-3: mild pain, 4-6: moderate pain, 7-10: severe pain.
Results and safety
This study is still under way. Results are not available in the source record yet.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 1 Oct 2026.
- Individual participant dataSharing planned: No. There is no plan to share individual participant data (IPD) with other researchers due to participant confidentiality and privacy considerations.
Related trials for Evaluation of Postoperative Pain and Antimicrobial Efficacy
Not yet recruitingPhase 4
Antibacterial Effect of Two Different Intracanal Medicaments and Their Effect on Post-operative Pain
Pulp Necrosis
Not yet recruiting
Evaluation of Postoperative Pain and Antimicrobial Efficacy of Selenium Versus Calcium Hydroxide As Intracanal Medication in Mandibular First Molars with Necrotic Pulp and Symptomatic Apical Periodontitis
Necrotic Pulp with Symptomatic Apical Periodontitis, Postoperative Dental Pain
Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing super-oxidized water (Microcyn) irrigating solution, 2% chlorhexidine irrigating solution and 2.5% sodium hypochlorite in people with Evaluation of Postoperative Pain and Antimicrobial Efficacy, run by Cairo University at 1 site in Egypt.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 6 inclusion and 5 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: opening soon, as of the sponsor's last update on 1 Oct 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Egypt. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
This study is still under way. Results are not available in the source record yet.
How can a doctor refer a patient or contact the research team?
Contact Paunceh S Soliman, PHD candidate (01026999750, paunceh.soliman@dentistry.cu.edu.eg), or a site near the patient. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Postoperative pain intensity at 6 hours post-instrumentation (time frame: 6 hours post-instrumentation). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 1 Oct 2026.