ExerciseAcute & BloodNCT07825740
Examination of Certain Blood Parameters Following Performance and Acute Exercise at Specific Times of the Day
A study testing Standardized Acute Exercise Protocol in people with Exercise and Physical Performance, run by Firat University at 1 site in Turkey (Türkiye).
Trial summary
This study aims to investigate whether the time of day at which acute exercise is performed influences physical performance and acute hormonal and biochemical responses in healthy young male soccer players. Read more about this study
- Who is it for?
- Ages 18 to 25 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Exercise, Physical Performance, Circadian Rhythm
- What is being tested?
- Standardized Acute Exercise Protocol
- Type of treatment
- Lifestyle or therapy programme
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 8 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 25 Nov 2025to 15 Jan 2026
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 25 years
- Men only
- Healthy volunteers accepted
- Adult
- Healthy male soccer players.
- Age between 18 and 25 years.
- Regular participation in organized soccer training.
- Free from musculoskeletal injury during the previous 6 months.
- Able and willing to provide written informed consent.
Trial locations and contacts
1 site in 1 country.
About this study
This study aims to investigate whether the time of day at which acute exercise is performed influences physical performance and acute hormonal and biochemical responses in healthy young male soccer players. Participants will complete the same standardized exercise protocol in the morning (08:00-09:30) and evening (17:00-18:30), with a 96-hour interval between sessions. Performance will be assessed using vertical jump and Yo-Yo shuttle run tests. Blood samples will be collected before exercise, immediately after exercise, and two hours after exercise to assess testosterone, cortisol, creatine kinase, AST, ALT, LDH, and troponin levels. The study will examine whether exercise responses differ between morning and evening sessions and whether these differences are consistent with circadian variation.
Read the detailed description
The purpose of this study is to examine the acute effects of exercise performed at different times of the day on physical performance, hormonal responses, and biochemical markers associated with exercise-induced muscle stress and recovery in healthy young male soccer players.
The study uses a single-group, repeated-measures pre-test/post-test design in which the same participants complete an identical standardized exercise protocol on two separate occasions. One exercise session will be performed in the morning between 08:00 and 09:30, and the other will be performed in the evening between 17:00 and 18:30. The two experimental sessions will be separated by 96 hours (4 days) to reduce the influence of the preceding exercise session on subsequent measurements.
Before each exercise session, participants will undergo anthropometric assessment and provide a resting blood sample. Participants will then complete standardized physical performance assessments, including a vertical jump test and a Yo-Yo shuttle run endurance test. Immediately after completion of the exercise protocol, a second blood sample will be collected. A third blood sample will be collected two hours after exercise to assess the early recovery response.
Blood samples will be analyzed for hormonal and biochemical parameters, including testosterone and cortisol as hormonal markers, and creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and troponin as biochemical markers of exercise-related physiological and metabolic responses.
The primary purpose of the comparison is to determine whether the acute physiological responses to the same exercise stimulus differ according to the time of day. The study will also explore the potential relationship between exercise timing and circadian variation in physical performance, hormonal responses, and biochemical markers.
All participants will complete the same exercise and measurement procedures under standardized conditions. The study population consists of healthy male soccer players who regularly participate in sports. Participants must be physically able to safely complete the exercise and assessment protocols and must not have medical or musculoskeletal conditions that could affect exercise performance or the safety of the study procedures.
This study is designed to provide information about the influence of exercise timing on acute performance and physiological responses and may contribute to the development of time-of-day considerations in exercise programming and sports training.
- Conditions
- Exercise, Physical Performance, Circadian Rhythm
- Being tested
- Standardized Acute Exercise Protocol
- Keywords
- Exercise1, Cortisol2, Creatine Kinase3, Circadian Rhythm4, Testosterone5,
- Medical subject headings
- Motor Activity
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Standardized Acute Exercise ProtocolBehavioural
Participants completed a standardized acute exercise protocol consisting of a vertical jump test and a Yo-Yo shuttle run endurance test. The protocol was performed twice, once in the morning (08:00-09:30) and once in the evening (17:00-18:30), with a 96-hour washout period between sessions. Blood samples were collected before exercise, immediately after exercise, and 2 hours after exercise to assess hormonal (testosterone and cortisol) and biochemical (CK, AST, ALT, LDH, and troponin) responses.
| Study group | Role | What they receive |
|---|---|---|
| Healthy Male Soccer Players | Experimental | Healthy male soccer players participated in a single-group intervention study. All participants completed the same standardized acute exercise protocol twice, once in the morning (08:00-09:30) and once in the evening (17:00-18:30), separated by 96 hours. Performance tests (vertical jump and Yo-Yo shuttle run) and blood samples were obtained before exercise, immediately after exercise, and 2 hours after exercise to evaluate hormonal and biochemical responses. Receives: Behavioral: Standardized Acute Exercise Protocol |
What is being measured
The primary outcome is the main question the study is designed to answer.
Yo-Yo Shuttle Run Performance
Change in endurance performance measured by the Yo-Yo shuttle run test following the standardized acute exercise protocol performed in the morning and evening.
Measured: Immediately after completion of the exercise performance assessment during each experimental session.
Secondary outcomes (8)
Vertical Jump Performance
Explosive lower-extremity performance will be assessed using the vertical jump test during the morning and evening experimental sessions.
Measured: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Testosterone Concentration
Serum testosterone concentration will be assessed from blood samples collected before exercise, immediately after exercise, and 2 hours after exercise during the morning and evening experimental sessions.
Measured: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Cortisol Concentration
Serum cortisol concentration will be assessed from blood samples collected before exercise, immediately after exercise, and 2 hours after exercise during the morning and evening experimental sessions.
Measured: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Creatine Kinase (CK) Concentration
Serum creatine kinase concentration will be assessed as a biochemical marker of exercise-related physiological response using blood samples collected before exercise, immediately after exercise, and 2 hours after exercise.
Measured: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Aspartate Aminotransferase (AST) Concentration
Serum AST concentration will be assessed from blood samples collected before exercise, immediately after exercise, and 2 hours after exercise during the morning and evening sessions.
Measured: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Alanine Aminotransferase (ALT) Concentration
Serum ALT concentration will be assessed from blood samples collected before exercise, immediately after exercise, and 2 hours after exercise during the morning and evening sessions.
Measured: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Lactate Dehydrogenase (LDH) Concentration
Serum LDH concentration will be assessed from blood samples collected before exercise, immediately after exercise, and 2 hours after exercise during the morning and evening sessions.
Measured: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Troponin Concentration
Serum troponin concentration will be assessed from blood samples collected before exercise, immediately after exercise, and 2 hours after exercise during the morning and evening experimental sessions.
Measured: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted17 Sep 2026
- Study start25 Nov 2025
- Primary completion15 Dec 2025
- Study completion15 Jan 2026
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for ExerciseStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Enrolment
- 8 participants (actual)
Sponsor and investigators
- Lead sponsor
- Firat University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Baha Engin ÇELİKEL, Firat University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07825740
- Sponsor's protocol ID
- 2025-25928
- Other IDs
- 2025
- Acronym
- Acute & Blood
- Status last verified
- Sep 2026
- First submitted
- 8 Jul 2026
- Last updated
- 17 Sep 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No: Individual participant data will not be made available to other researchers. The study dataset will be used for the purposes described in the study protocol and will not be shared at the individual participant level.
Study at a glance
From the registry record, last updated by the sponsor on 17 Sep 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Examination of Certain Blood Parameters Following Performance and Acute Exercise at Specific Times of the Day
- ClinicalTrials.gov ID
- NCT07825740
- Acronym / sponsor ID
- Acute & Blood / 2025-25928
- Conditions
- Exercise, Physical Performance, Circadian Rhythm
- Keywords
- Exercise1, Cortisol2, Creatine Kinase3, Circadian Rhythm4, Testosterone5,
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 8 participants (actual)
- Lead sponsor
- Firat UniversityUniversity, hospital or other
- Study start
- 25 Nov 2025 (actual)
- Primary completion
- 15 Dec 2025 (actual)
- Study completion
- 15 Jan 2026 (actual)
- First posted
- 17 Sep 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
This study aims to investigate whether the time of day at which acute exercise is performed influences physical performance and acute hormonal and biochemical responses in healthy young male soccer players. Participants will complete the same standardized exercise protocol in the morning (08:00-09:30) and evening (17:00-18:30), with a 96-hour interval between sessions. Performance will be assessed using vertical jump and Yo-Yo shuttle run tests. Blood samples will be collected before exercise, immediately after exercise, and two hours after exercise to assess testosterone, cortisol, creatine kinase, AST, ALT, LDH, and troponin levels. The study will examine whether exercise responses differ between morning and evening sessions and whether these differences are consistent with circadian variation.
Detailed description
The purpose of this study is to examine the acute effects of exercise performed at different times of the day on physical performance, hormonal responses, and biochemical markers associated with exercise-induced muscle stress and recovery in healthy young male soccer players.
The study uses a single-group, repeated-measures pre-test/post-test design in which the same participants complete an identical standardized exercise protocol on two separate occasions. One exercise session will be performed in the morning between 08:00 and 09:30, and the other will be performed in the evening between 17:00 and 18:30. The two experimental sessions will be separated by 96 hours (4 days) to reduce the influence of the preceding exercise session on subsequent measurements.
Before each exercise session, participants will undergo anthropometric assessment and provide a resting blood sample. Participants will then complete standardized physical performance assessments, including a vertical jump test and a Yo-Yo shuttle run endurance test. Immediately after completion of the exercise protocol, a second blood sample will be collected. A third blood sample will be collected two hours after exercise to assess the early recovery response.
Blood samples will be analyzed for hormonal and biochemical parameters, including testosterone and cortisol as hormonal markers, and creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and troponin as biochemical markers of exercise-related physiological and metabolic responses.
The primary purpose of the comparison is to determine whether the acute physiological responses to the same exercise stimulus differ according to the time of day. The study will also explore the potential relationship between exercise timing and circadian variation in physical performance, hormonal responses, and biochemical markers.
All participants will complete the same exercise and measurement procedures under standardized conditions. The study population consists of healthy male soccer players who regularly participate in sports. Participants must be physically able to safely complete the exercise and assessment protocols and must not have medical or musculoskeletal conditions that could affect exercise performance or the safety of the study procedures.
This study is designed to provide information about the influence of exercise timing on acute performance and physiological responses and may contribute to the development of time-of-day considerations in exercise programming and sports training.
Study design
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Number of arms
- 1
| Arm | Type | Receives |
|---|---|---|
| Healthy Male Soccer Players | Experimental | Behavioral: Standardized Acute Exercise ProtocolHealthy male soccer players participated in a single-group intervention study. All participants completed the same standardized acute exercise protocol twice, once in the morning (08:00-09:30) and once in the evening (17:00-18:30), separated by 96 hours. Performance tests (vertical jump and Yo-Yo shuttle run) and blood samples were obtained before exercise, immediately after exercise, and 2 hours after exercise to evaluate hormonal and biochemical responses. |
Who may qualify
- Ages
- 18 to 25 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Standardized Acute Exercise ProtocolParticipants completed a standardized acute exercise protocol consisting of a vertical jump test and a Yo-Yo shuttle run endurance test. The protocol was performed twice, once in the morning (08:00-09:30) and once in the evening (17:00-18:30), with a 96-hour washout period between sessions. Blood samples were collected before exercise, immediately after exercise, and 2 hours after exercise to assess hormonal (testosterone and cortisol) and biochemical (CK, AST, ALT, LDH, and troponin) responses. | Behavioural | Healthy Male Soccer Players |
Primary outcome
Yo-Yo Shuttle Run Performance
Time frame: Immediately after completion of the exercise performance assessment during each experimental session.
Change in endurance performance measured by the Yo-Yo shuttle run test following the standardized acute exercise protocol performed in the morning and evening.
8 secondary outcomes
Vertical Jump Performance
Time frame: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Testosterone Concentration
Time frame: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Cortisol Concentration
Time frame: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Creatine Kinase (CK) Concentration
Time frame: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Aspartate Aminotransferase (AST) Concentration
Time frame: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Alanine Aminotransferase (ALT) Concentration
Time frame: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Lactate Dehydrogenase (LDH) Concentration
Time frame: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise.
Serum Troponin Concentration
Time frame: Baseline (pre-exercise), immediately post-exercise, and 2 hours post-exercise
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 17 Sep 2026.
- Individual participant dataSharing planned: No. Individual participant data will not be made available to other researchers. The study dataset will be used for the purposes described in the study protocol and will not be shared at the individual participant level.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Standardized Acute Exercise Protocol in people with Exercise and Physical Performance, run by Firat University at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 17 Sep 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Yo-Yo Shuttle Run Performance (time frame: Immediately after completion of the exercise performance assessment during each experimental session.). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 17 Sep 2026.