Sedentary BehaviorAERAENCT07823335
Acute Endocrine Responses to Aerobic and Anaerobic Exercise in Sedentary Women: A Comparative Analysis of Thyroid Hormones and Cortisol
A study testing 20-Meter Shuttle Run Test and Wingate Anaerobic Test in people with Sedentary Behavior and Acute Exercise, run by Assoc. Prof. Dr. Özgür EKEN at 1 site in Turkey (Türkiye).
Trial summary
This study aims to compare acute thyroid hormone and cortisol responses to aerobic and anaerobic exercise in sedentary women. Thirty sedentary women aged 18-25 years will voluntarily participate in the study. Read more about this study
- Who is it for?
- Ages 18 to 24 years, women onlyHealthy volunteers can take part
- Conditions the study is for
- Sedentary Behavior, Acute Exercise, Endocrine Response
- What is being tested?
- 20-Meter Shuttle Run Test and Wingate Anaerobic Test
- Type of treatment
- Lifestyle or therapy programme
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 14 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 30 Oct 2026to 15 Nov 2026
- Phase
- Interventional
- Are there results?
- Not yet: the study is under way
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 24 years
- Women only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Female participants aged 18-25 years
- Studying at the Faculty of Sports Sciences of Yalova University
- Not actively participating in regular exercise or sports training
- Voluntarily agreeing to participate in the study
- Providing written informed consent
- Being medically eligible to perform the exercise protocols following preparticipation medical screening
Who cannot join
- Having a neurological disorder during the previous year
- Having an auditory, visual, or vestibular disorder during the previous year
- Having experienced a serious upper- or lower-extremity injury during the previous six months
- Having any medical condition identified during screening that could prevent safe participation in maximal exercise testing
- Being unable or unwilling to complete either exercise protocol or blood-sampling procedures
Trial locations and contacts
1 site in 1 country. Contact the study team or a site near you to ask about taking part.
Turkey (Türkiye)1 site, 1 recruiting
- Recruiting
Karabuk University, Hasan Dogan Faculty of Sport Sciences
Karabük, Karabük Province, 78050
Musa ŞAHİN, PhD, +905549755058, musasahin@karabuk.edu.tr
About this study
This study aims to compare acute thyroid hormone and cortisol responses to aerobic and anaerobic exercise in sedentary women. Thirty sedentary women aged 18-25 years will voluntarily participate in the study. Participants will complete a 20-meter shuttle run test as the aerobic exercise protocol and a 30-second Wingate test as the anaerobic exercise protocol in separate testing sessions. Blood samples will be collected immediately before and after each exercise protocol. Serum thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and cortisol concentrations will be determined using chemiluminescence-based laboratory methods. Changes in hormone concentrations will be compared between the aerobic and anaerobic exercise conditions.
Read the detailed description
This interventional study is designed to compare the acute endocrine responses elicited by aerobic and anaerobic exercise in sedentary women. A total of 30 healthy sedentary women aged 18-25 years will voluntarily participate. Individuals who have engaged in regular exercise or sports training, experienced a serious upper- or lower-extremity injury during the previous six months, or had a neurological, auditory, visual, or vestibular disorder during the previous year will not be included. Before participation, all individuals will be informed about the study procedures, provide written informed consent, and undergo medical screening.
Each participant will complete aerobic and anaerobic exercise protocols in separate testing sessions. The aerobic exercise protocol will consist of a progressive 20-meter shuttle run test. Participants will run continuously between two lines positioned 20 meters apart, following prerecorded audio signals. Running speed will progressively increase as the interval between signals decreases. The test will end when a participant fails to reach the designated line before the signal on two consecutive occasions.
The anaerobic exercise protocol will consist of a 30-second Wingate test performed on a Monark cycle ergometer connected to computerized analysis software. Following an unloaded acceleration period of approximately 3-4 seconds, an external resistance equivalent to 65 g/kg of body mass will be applied. Participants will be instructed to pedal against this resistance at maximal effort for 30 seconds.
Four venous blood samples will be collected from each participant: immediately before and after the aerobic exercise protocol and immediately before and after the anaerobic exercise protocol. Each sample will consist of approximately 8 mL of blood collected from an antecubital vein by qualified healthcare professionals under medical supervision. The samples will be centrifuged at 3,500 revolutions per minute to separate serum and plasma. Serum thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and cortisol concentrations will be measured using chemiluminescence-based laboratory methods.
Exercise testing will be conducted at the Hasan Doğan School of Physical Education and Sports Laboratory at Karabuk University. Laboratory analyses will be performed at the Public Health Laboratory of the Karabuk Provincial Health Directorate. Pre-to-post-exercise changes in hormone concentrations will be evaluated within each exercise condition and compared between the aerobic and anaerobic exercise protocols.
- Conditions
- Sedentary Behavior, Acute Exercise, Endocrine Response
- Being tested
- 20-Meter Shuttle Run Test, Wingate Anaerobic Test
- Keywords
- Aerobic Exercise, Anaerobic Exercise, Sedentary Women, Cortisol, Thyroid Hormones
- Medical subject headings
- Sedentary Behavior
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
20-Meter Shuttle Run TestBehavioural
Participants will perform a progressive 20-meter shuttle run test by running continuously between two lines positioned 20 meters apart in time with prerecorded audio signals. The initial running speed will progressively increase as the interval between audio signals decreases. The test will end when the participant fails to reach the designated line before the signal on two consecutive occasions.
Wingate Anaerobic TestBehavioural
Participants will perform a 30-second Wingate anaerobic test on a Monark cycle ergometer connected to computerized analysis software. Following an unloaded acceleration phase of approximately 3-4 seconds, a resistance corresponding to 65 g/kg of body mass will be applied. Participants will be instructed to pedal against this resistance at maximal effort for 30 seconds. Peak power, mean power, minimum power, and fatigue index will be recorded.
| Study group | Role | What they receive |
|---|---|---|
| Aerobic and Anaerobic Exercise | Experimental | Participants will complete both a progressive 20-meter shuttle run test and a 30-second Wingate anaerobic test in separate testing sessions. Venous blood samples will be collected immediately before and after each exercise protocol to assess acute changes in serum thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and cortisol concentrations. Each participant will serve as her own control. Receives: Behavioral: 20-Meter Shuttle Run Test; Behavioral: Wingate Anaerobic Test |
What is being measured
The primary outcome is the main question the study is designed to answer.
Change in Serum Cortisol Concentration
Measured: Immediately before and immediately after each exercise protocol
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted16 Sep 2026
- Study start30 Oct 2026 (estimated)
- Primary completion1 Nov 2026 (estimated)
- Study completion15 Nov 2026 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Sedentary BehaviorStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Model description
- Each participant will complete both the aerobic and anaerobic exercise interventions in two separate testing sessions and will serve as her own control. Acute changes in thyroid hormone and cortisol concentrations from before to after each exercise protocol will be compared between the two exercise conditions.
- Masking
- None (open label)
- Enrolment
- 14 participants (estimated)
Sponsor and investigators
- Lead sponsor
- Assoc. Prof. Dr. Özgür EKEN
- Sponsor type
- University, hospital or other
- Collaborators
- Karabuk University
- Responsible party
- Sponsor-investigator, Assoc. Prof. Dr. Özgür EKEN, Inonu University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07823335
- Sponsor's protocol ID
- KBU-THYROID-2026-01
- Acronym
- AERAE
- Status last verified
- Oct 2026
- First submitted
- 11 Sep 2026
- Last updated
- 27 Oct 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No: Individual participant data will not be shared because no data-sharing plan was included in the approved study protocol or informed consent process.
Study at a glance
From the registry record, last updated by the sponsor on 27 Oct 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Acute Endocrine Responses to Aerobic and Anaerobic Exercise in Sedentary Women: A Comparative Analysis of Thyroid Hormones and Cortisol
- ClinicalTrials.gov ID
- NCT07823335
- Acronym / sponsor ID
- AERAE / KBU-THYROID-2026-01
- Conditions
- Sedentary Behavior, Acute Exercise, Endocrine Response
- Keywords
- Aerobic Exercise, Anaerobic Exercise, Sedentary Women, Cortisol, Thyroid Hormones
- Study type
- Interventional
- Recruitment status
- Recruiting
- Enrolment
- 14 participants (estimated)
- Lead sponsor
- Assoc. Prof. Dr. Özgür EKENUniversity, hospital or other
- Collaborators
- Karabuk University
- Study start
- 30 Oct 2026 (estimated)
- Primary completion
- 1 Nov 2026 (estimated)
- Study completion
- 15 Nov 2026 (estimated)
- First posted
- 16 Sep 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
This study aims to compare acute thyroid hormone and cortisol responses to aerobic and anaerobic exercise in sedentary women. Thirty sedentary women aged 18-25 years will voluntarily participate in the study. Participants will complete a 20-meter shuttle run test as the aerobic exercise protocol and a 30-second Wingate test as the anaerobic exercise protocol in separate testing sessions. Blood samples will be collected immediately before and after each exercise protocol. Serum thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and cortisol concentrations will be determined using chemiluminescence-based laboratory methods. Changes in hormone concentrations will be compared between the aerobic and anaerobic exercise conditions.
Detailed description
This interventional study is designed to compare the acute endocrine responses elicited by aerobic and anaerobic exercise in sedentary women. A total of 30 healthy sedentary women aged 18-25 years will voluntarily participate. Individuals who have engaged in regular exercise or sports training, experienced a serious upper- or lower-extremity injury during the previous six months, or had a neurological, auditory, visual, or vestibular disorder during the previous year will not be included. Before participation, all individuals will be informed about the study procedures, provide written informed consent, and undergo medical screening.
Each participant will complete aerobic and anaerobic exercise protocols in separate testing sessions. The aerobic exercise protocol will consist of a progressive 20-meter shuttle run test. Participants will run continuously between two lines positioned 20 meters apart, following prerecorded audio signals. Running speed will progressively increase as the interval between signals decreases. The test will end when a participant fails to reach the designated line before the signal on two consecutive occasions.
The anaerobic exercise protocol will consist of a 30-second Wingate test performed on a Monark cycle ergometer connected to computerized analysis software. Following an unloaded acceleration period of approximately 3-4 seconds, an external resistance equivalent to 65 g/kg of body mass will be applied. Participants will be instructed to pedal against this resistance at maximal effort for 30 seconds.
Four venous blood samples will be collected from each participant: immediately before and after the aerobic exercise protocol and immediately before and after the anaerobic exercise protocol. Each sample will consist of approximately 8 mL of blood collected from an antecubital vein by qualified healthcare professionals under medical supervision. The samples will be centrifuged at 3,500 revolutions per minute to separate serum and plasma. Serum thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and cortisol concentrations will be measured using chemiluminescence-based laboratory methods.
Exercise testing will be conducted at the Hasan Doğan School of Physical Education and Sports Laboratory at Karabuk University. Laboratory analyses will be performed at the Public Health Laboratory of the Karabuk Provincial Health Directorate. Pre-to-post-exercise changes in hormone concentrations will be evaluated within each exercise condition and compared between the aerobic and anaerobic exercise protocols.
Study design
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Number of arms
- 1
- Model description
- Each participant will complete both the aerobic and anaerobic exercise interventions in two separate testing sessions and will serve as her own control. Acute changes in thyroid hormone and cortisol concentrations from before to after each exercise protocol will be compared between the two exercise conditions.
| Arm | Type | Receives |
|---|---|---|
| Aerobic and Anaerobic Exercise | Experimental | Behavioral: 20-Meter Shuttle Run Test, Behavioral: Wingate Anaerobic TestParticipants will complete both a progressive 20-meter shuttle run test and a 30-second Wingate anaerobic test in separate testing sessions. Venous blood samples will be collected immediately before and after each exercise protocol to assess acute changes in serum thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4), and cortisol concentrations. Each participant will serve as her own control. |
Who may qualify
- Ages
- 18 to 24 years
- Sex
- Female only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (6 listed)
- Female participants aged 18-25 years
- Studying at the Faculty of Sports Sciences of Yalova University
- Not actively participating in regular exercise or sports training
- Voluntarily agreeing to participate in the study
- Providing written informed consent
Key exclusion criteria (5 listed)
- Having a neurological disorder during the previous year
- Having an auditory, visual, or vestibular disorder during the previous year
- Having experienced a serious upper- or lower-extremity injury during the previous six months
- Having any medical condition identified during screening that could prevent safe participation in maximal exercise testing
- Being unable or unwilling to complete either exercise protocol or blood-sampling procedures
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| 20-Meter Shuttle Run TestParticipants will perform a progressive 20-meter shuttle run test by running continuously between two lines positioned 20 meters apart in time with prerecorded audio signals. The initial running speed will progressively increase as the interval between audio signals decreases. The test will end when the participant fails to reach the designated line before the signal on two consecutive occasions. | Behavioural | Aerobic and Anaerobic Exercise |
| Wingate Anaerobic TestParticipants will perform a 30-second Wingate anaerobic test on a Monark cycle ergometer connected to computerized analysis software. Following an unloaded acceleration phase of approximately 3-4 seconds, a resistance corresponding to 65 g/kg of body mass will be applied. Participants will be instructed to pedal against this resistance at maximal effort for 30 seconds. Peak power, mean power, minimum power, and fatigue index will be recorded. | Behavioural | Aerobic and Anaerobic Exercise |
Primary outcome
Change in Serum Cortisol Concentration
Time frame: Immediately before and immediately after each exercise protocol
Results and safety
This study is still under way. Results are not available in the source record yet.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 27 Oct 2026.
- Individual participant dataSharing planned: No. Individual participant data will not be shared because no data-sharing plan was included in the approved study protocol or informed consent process.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing 20-Meter Shuttle Run Test and Wingate Anaerobic Test in people with Sedentary Behavior and Acute Exercise, run by Assoc. Prof. Dr. Özgür EKEN at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 6 inclusion and 5 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: looking for participants now, as of the sponsor's last update on 27 Oct 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
This study is still under way. Results are not available in the source record yet.
How can a doctor refer a patient or contact the research team?
Contact Özgür EKEN, PhD (+905532132795, ozgur.eken@inonu.edu.tr), or a site near the patient. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Change in Serum Cortisol Concentration (time frame: Immediately before and immediately after each exercise protocol). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 27 Oct 2026.