Oxidative StressNCT07662954
Effects of Magnesium and Spinning Training on Echocardiographic, Inflammatory, and Aerobic Outcomes
A study testing Spinning Training and Magnesium Supplementation in people with Oxidative Stress and Inflammation, run by Karamanoğlu Mehmetbey University at 1 site in Turkey (Türkiye).
Trial summary
Regular aerobic exercise may improve cardiorespiratory fitness while modulating systemic inflammation and oxidative stress. Spinning, as a structured indoor cycling modality, provides a practical aerobic exercise model with adjustable intensity and duration, but its effects on redox balance, inflammatory status… Read more about this study
- Who is it for?
- Ages 18 to 30 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Oxidative Stress, Inflammation, Physical Fitness
- What is being tested?
- Spinning Training and Magnesium Supplementation
- Type of treatment
- Pill or oral medicine, Lifestyle or therapy programme
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 48 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 1 Jun 2026to 20 Aug 2026
- Phase
- Interventional
- Are there results?
- Not yet: the study is under way
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 30 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Self-identified male participants
- Aged 18 to 30 years
- Physically active individuals
- Participants who are eligible to participate in a structured spinning training program
- Participants with available baseline and follow-up assessments for biochemical, aerobic capacity, and cardiac function parameters
Who cannot join
- Presence of any chronic systemic disease
- Acute infection during the study period
- Regular use of anti-inflammatory or antioxidant medications
- Use of antioxidant or ergogenic supplements other than magnesium
- Any cardiovascular, musculoskeletal, or neurological condition that may limit participation in spinning training
- Missing baseline or follow-up outcome data
Trial locations and contacts
1 site in 1 country. Contact the study team or a site near you to ask about taking part.
Turkey (Türkiye)1 site
Karamanoğlu Mehmetbey University, Faculty of Sport Sciences
Karaman, 70100
About this study
Official title: Effects of Magnesium Supplementation and Spinning Training on Cardiac Function, Oxidative Stress, Inflammation, and Aerobic Capacity
Regular aerobic exercise may improve cardiorespiratory fitness while modulating systemic inflammation and oxidative stress. Spinning, as a structured indoor cycling modality, provides a practical aerobic exercise model with adjustable intensity and duration, but its effects on redox balance, inflammatory status, aerobic capacity, and cardiac functional parameters may vary according to individual recovery and nutritional status. Magnesium is an essential micronutrient involved in energy metabolism, muscle contraction-relaxation, ion regulation, and inflammatory and oxidative pathways; therefore, magnesium use may be relevant to exercise adaptation and recovery.
This study aims to evaluate the associations of magnesium use and spinning training with oxidative stress, inflammation, aerobic capacity, and cardiac parameters. In this context, biochemical markers related to oxidative/antioxidant status and inflammation, aerobic performance indicators, and echocardiographic cardiac function parameters will be assessed together. The study is expected to provide real-world evidence on whether magnesium use in individuals participating in spinning training is associated with more favorable redox, inflammatory, aerobic, and cardiac profiles.
- Conditions
- Oxidative Stress, Inflammation, Physical Fitness, Echocardiography, Exercise, Healthy Volunteers
- Being tested
- Spinning Training, Magnesium Supplementation
- Medical subject headings
- Inflammation, Motor Activity
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Spinning TrainingBehavioural
Participants will participate in a structured spinning training program during the study period. The program will consist of supervised indoor cycling sessions performed at a planned frequency, duration, and intensity.
Magnesium SupplementationDietary supplement
Participants will receive oral magnesium supplementation during the study period in addition to the structured spinning training program.
| Study group | Role | What they receive |
|---|---|---|
| Control group | No intervention | Participants in this arm will not receive any exercise training, magnesium supplementation, or placebo intervention during the study period. They will continue their usual daily routines and will undergo the same baseline and follow-up assessments as the intervention groups. |
| Spinning Training Group | Active comparator | Participants in this arm will participate in a structured spinning training program during the study period. No magnesium supplementation or placebo will be administered. Baseline and follow-up assessments will include oxidative stress, inflammatory, aerobic capacity, and cardiac function parameters. Receives: Behavioral: Spinning Training |
| Spinning + Magnesium Group | Experimental | Participants in this arm will participate in a structured spinning training program and receive magnesium supplementation during the study period. Baseline and follow-up assessments will include oxidative stress, inflammatory, aerobic capacity, and cardiac function parameters. Receives: Behavioral: Spinning Training; Dietary Supplement: Magnesium Supplementation |
What is being measured
The primary outcome is the main question the study is designed to answer.
Change in Maximal Oxygen Uptake (VO₂max)
Maximal oxygen uptake (VO₂max, mL/kg/min) will be assessed using a graded exercise test. The change in VO₂max from baseline to 6 weeks will be evaluated.
Measured: Baseline and 6 weeks
Change in Serum Magnesium Concentration
Serum magnesium concentration (mg/dL) will be measured using standard laboratory methods. The change in serum magnesium levels from baseline to 6 weeks will be evaluated.
Measured: Baseline and 6 weeks
Secondary outcomes (11)
Change in Total Antioxidant Status (TAS)
Serum total antioxidant status (TAS, mmol Trolox equivalent/L) will be measured using commercially available assay kits. The change in TAS from baseline to 6 weeks will be evaluated.
Measured: Baseline and 6 weeks
Change in Total Oxidant Status (TOS)
Serum total oxidant status (TOS, μmol H₂O₂ equivalent/L) will be measured using commercially available assay kits. The change in TOS from baseline to 6 weeks will be evaluated.
Measured: Baseline and 6 weeks
Change in Paraoxonase-1 Activity (PON1)
Serum paraoxonase-1 (PON1, U/L) activity will be determined using spectrophotometric methods. The change in PON1 activity from baseline to 6 weeks will be evaluated.
Measured: Baseline and 6 weeks
Change in Arylesterase Activity (ARE)
Serum arylesterase (ARE, kU/L) activity will be determined using spectrophotometric methods. The change in ARE activity from baseline to 6 weeks will be evaluated.
Measured: Baseline and 6 weeks
Change in High-Sensitivity C-Reactive Protein (hs-CRP)
Serum high-sensitivity C-reactive protein (hs-CRP, mg/L) concentration will be measured using standard laboratory methods. The change in hs-CRP levels from baseline to 6 weeks will be evaluated.
Measured: Baseline and 6 weeks
Change in Interleukin-6 (IL-6)
Serum interleukin-6 (IL-6, pg/mL) concentration will be measured using enzyme-linked immunosorbent assay methods. The change in IL-6 levels from baseline to 6 weeks will be evaluated.
Measured: Baseline and 6 weeks
Change in Tumor Necrosis Factor-Alpha (TNF-α)
Serum tumor necrosis factor-alpha (TNF-α, pg/mL) concentration will be measured using enzyme-linked immunosorbent assay methods. The change in TNF-α levels from baseline to 6 weeks will be evaluated.
Measured: Baseline and 6 weeks
Change in Serum Potassium Concentration
Serum potassium concentration (mmol/L) will be measured using standard biochemical methods. The change in serum potassium levels from baseline to 6 weeks will be evaluated.
Measured: Baseline and 6 weeks
Change in Serum Calcium Concentration
Serum calcium concentration (mg/dL) will be measured using standard biochemical methods. The change in serum calcium levels from baseline to 6 weeks will be evaluated.
Measured: Baseline and 6 weeks
Change in N-Terminal Pro-B-Type Natriuretic Peptide (NT-proBNP)
Serum N-terminal pro-B-type natriuretic peptide (NT-proBNP, pg/mL) concentration will be measured using immunoassay methods. The change in NT-proBNP levels from baseline to 6 weeks will be evaluated.
Measured: Baseline and 6 weeks
Change in Left Ventricular Ejection Fraction (LVEF)
Left ventricular ejection fraction (LVEF, %) will be assessed by transthoracic echocardiography. The change in LVEF from baseline to 6 weeks will be evaluated.
Measured: Baseline and 6 weeks
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted23 Jun 2026
- Study start1 Jun 2026
- Primary completion15 Aug 2026 (estimated)
- Study completion20 Aug 2026 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Publications (7)Papers the sponsor has linked to this study.
- Other trials for Oxidative StressStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Single blind
- Who is masked
- Outcomes assessor
- Enrolment
- 48 participants (estimated)
Sponsor and investigators
- Lead sponsor
- Karamanoğlu Mehmetbey University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Hikmet Kocaman, Karamanoğlu Mehmetbey University
- Investigators
- Şemsi Gül Yılmaz Kocaman, PhD, Principal investigator, Karamanoğlu Mehmetbey University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07662954
- Sponsor's protocol ID
- 02-2026/29
- Status last verified
- Jun 2026
- First submitted
- 5 Jun 2026
- Last updated
- 23 Jun 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No: Individual participant data will not be shared because the dataset contains participant-level health and performance records. All results will be reported in aggregate form, and confidentiality will be maintained in accordance with applicable data protection regulations.
Publications
Tarsitano MG, Quinzi F, Folino K, Greco F, Oranges FP, Cerulli C, Emerenziani GP. Effects of magnesium supplementation on muscle soreness in different type of physical activities: a systematic review. J Transl Med. 2024 Jul 5;22(1):629. doi: 10.1186/s12967-024-05434-x.
PubMed 38970118Sireno L, Dimauro I, Caporossi D. Reactive oxygen species in exercise biology: from adaptive stress response to cell signaling and beyond. Free Radic Biol Med. 2026 Mar 1;245:447-462. doi: 10.1016/j.freeradbiomed.2025.12.044. Epub 2026 Jan 2. No abstract available.
PubMed 41485544Reno AM, Green M, Killen LG, O'Neal EK, Pritchett K, Hanson Z. Effects of Magnesium Supplementation on Muscle Soreness and Performance. J Strength Cond Res. 2022 Aug 1;36(8):2198-2203. doi: 10.1519/JSC.0000000000003827. Epub 2020 Oct 1.
PubMed 33009349Feng W, Wang Y, Gu X, Yu D, Liu Z. Exercise as a modulator of systemic inflammation and oxidative stress biomarkers across clinical and healthy populations: an umbrella meta-analysis. BMC Sports Sci Med Rehabil. 2025 Nov 28;17(1):360. doi: 10.1186/s13102-025-01327-8.
PubMed 41316311Dominguez LJ, Veronese N, Ragusa FS, Baio SM, Sgro F, Russo A, Battaglia G, Bianco A, Barbagallo M. The Importance of Vitamin D and Magnesium in Athletes. Nutrients. 2025 May 13;17(10):1655. doi: 10.3390/nu17101655.
PubMed 40431395Chavarrias M, Carlos-Vivas J, Collado-Mateo D, Perez-Gomez J. Health Benefits of Indoor Cycling: A Systematic Review. Medicina (Kaunas). 2019 Aug 8;55(8):452. doi: 10.3390/medicina55080452.
PubMed 31443139Battista RA, Foster C, Andrew J, Wright G, Lucia A, Porcari JP. Physiologic responses during indoor cycling. J Strength Cond Res. 2008 Jul;22(4):1236-41. doi: 10.1519/JSC.0b013e318173dbc4.
PubMed 18545183
Study at a glance
From the registry record, last updated by the sponsor on 23 Jun 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Effects of Magnesium Supplementation and Spinning Training on Cardiac Function, Oxidative Stress, Inflammation, and Aerobic Capacity
- ClinicalTrials.gov ID
- NCT07662954
- Acronym / sponsor ID
- 02-2026/29
- Conditions
- Oxidative Stress, Inflammation, Physical Fitness, Echocardiography, Exercise, Healthy Volunteers
- Study type
- Interventional
- Recruitment status
- Enrolling by invitation
- Enrolment
- 48 participants (estimated)
- Lead sponsor
- Karamanoğlu Mehmetbey UniversityUniversity, hospital or other
- Study start
- 1 Jun 2026 (actual)
- Primary completion
- 15 Aug 2026 (estimated)
- Study completion
- 20 Aug 2026 (estimated)
- First posted
- 23 Jun 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Regular aerobic exercise may improve cardiorespiratory fitness while modulating systemic inflammation and oxidative stress. Spinning, as a structured indoor cycling modality, provides a practical aerobic exercise model with adjustable intensity and duration, but its effects on redox balance, inflammatory status, aerobic capacity, and cardiac functional parameters may vary according to individual recovery and nutritional status. Magnesium is an essential micronutrient involved in energy metabolism, muscle contraction-relaxation, ion regulation, and inflammatory and oxidative pathways; therefore, magnesium use may be relevant to exercise adaptation and recovery.
This study aims to evaluate the associations of magnesium use and spinning training with oxidative stress, inflammation, aerobic capacity, and cardiac parameters. In this context, biochemical markers related to oxidative/antioxidant status and inflammation, aerobic performance indicators, and echocardiographic cardiac function parameters will be assessed together. The study is expected to provide real-world evidence on whether magnesium use in individuals participating in spinning training is associated with more favorable redox, inflammatory, aerobic, and cardiac profiles.
Study design
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Single blind (outcomes assessor)
- Number of arms
- 3
| Arm | Type | Receives |
|---|---|---|
| Control group | No intervention | –Participants in this arm will not receive any exercise training, magnesium supplementation, or placebo intervention during the study period. They will continue their usual daily routines and will undergo the same baseline and follow-up assessments as the intervention groups. |
| Spinning Training Group | Active comparator | Behavioral: Spinning TrainingParticipants in this arm will participate in a structured spinning training program during the study period. No magnesium supplementation or placebo will be administered. Baseline and follow-up assessments will include oxidative stress, inflammatory, aerobic capacity, and cardiac function parameters. |
| Spinning + Magnesium Group | Experimental | Behavioral: Spinning Training, Dietary Supplement: Magnesium SupplementationParticipants in this arm will participate in a structured spinning training program and receive magnesium supplementation during the study period. Baseline and follow-up assessments will include oxidative stress, inflammatory, aerobic capacity, and cardiac function parameters. |
Who may qualify
- Ages
- 18 to 30 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (5 listed)
- Self-identified male participants
- Aged 18 to 30 years
- Physically active individuals
- Participants who are eligible to participate in a structured spinning training program
- Participants with available baseline and follow-up assessments for biochemical, aerobic capacity, and cardiac function parameters
Key exclusion criteria (6 listed)
- Presence of any chronic systemic disease
- Acute infection during the study period
- Regular use of anti-inflammatory or antioxidant medications
- Use of antioxidant or ergogenic supplements other than magnesium
- Any cardiovascular, musculoskeletal, or neurological condition that may limit participation in spinning training
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Spinning TrainingParticipants will participate in a structured spinning training program during the study period. The program will consist of supervised indoor cycling sessions performed at a planned frequency, duration, and intensity. | Behavioural | Spinning + Magnesium Group, Spinning Training Group |
| Magnesium SupplementationParticipants will receive oral magnesium supplementation during the study period in addition to the structured spinning training program. | Dietary supplement | Spinning + Magnesium Group |
Primary outcome
Change in Maximal Oxygen Uptake (VO₂max)
Time frame: Baseline and 6 weeks
Maximal oxygen uptake (VO₂max, mL/kg/min) will be assessed using a graded exercise test. The change in VO₂max from baseline to 6 weeks will be evaluated.
Change in Serum Magnesium Concentration
Time frame: Baseline and 6 weeks
Serum magnesium concentration (mg/dL) will be measured using standard laboratory methods. The change in serum magnesium levels from baseline to 6 weeks will be evaluated.
11 secondary outcomes
Change in Total Antioxidant Status (TAS)
Time frame: Baseline and 6 weeks
Change in Total Oxidant Status (TOS)
Time frame: Baseline and 6 weeks
Change in Paraoxonase-1 Activity (PON1)
Time frame: Baseline and 6 weeks
Change in Arylesterase Activity (ARE)
Time frame: Baseline and 6 weeks
Change in High-Sensitivity C-Reactive Protein (hs-CRP)
Time frame: Baseline and 6 weeks
Change in Interleukin-6 (IL-6)
Time frame: Baseline and 6 weeks
Change in Tumor Necrosis Factor-Alpha (TNF-α)
Time frame: Baseline and 6 weeks
Change in Serum Potassium Concentration
Time frame: Baseline and 6 weeks
Change in Serum Calcium Concentration
Time frame: Baseline and 6 weeks
Change in N-Terminal Pro-B-Type Natriuretic Peptide (NT-proBNP)
Time frame: Baseline and 6 weeks
Change in Left Ventricular Ejection Fraction (LVEF)
Time frame: Baseline and 6 weeks
Results and safety
This study is still under way. Results are not available in the source record yet.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 23 Jun 2026.
- Background: PubMed 38970118Tarsitano MG, Quinzi F, Folino K, Greco F, Oranges FP, Cerulli C, Emerenziani GP. Effects of magnesium supplementation on muscle soreness in different type of physical activities: a systematic review. J Transl Med. 2024 Jul 5;22(1):629. doi: 10.1186/s12967-024-05434-x.
- Background: PubMed 41485544Sireno L, Dimauro I, Caporossi D. Reactive oxygen species in exercise biology: from adaptive stress response to cell signaling and beyond. Free Radic Biol Med. 2026 Mar 1;245:447-462. doi: 10.1016/j.freeradbiomed.2025.12.044. Epub 2026 Jan 2. No abstract available.
- Background: PubMed 33009349Reno AM, Green M, Killen LG, O'Neal EK, Pritchett K, Hanson Z. Effects of Magnesium Supplementation on Muscle Soreness and Performance. J Strength Cond Res. 2022 Aug 1;36(8):2198-2203. doi: 10.1519/JSC.0000000000003827. Epub 2020 Oct 1.
- Background: PubMed 41316311Feng W, Wang Y, Gu X, Yu D, Liu Z. Exercise as a modulator of systemic inflammation and oxidative stress biomarkers across clinical and healthy populations: an umbrella meta-analysis. BMC Sports Sci Med Rehabil. 2025 Nov 28;17(1):360. doi: 10.1186/s13102-025-01327-8.
- Background: PubMed 40431395Dominguez LJ, Veronese N, Ragusa FS, Baio SM, Sgro F, Russo A, Battaglia G, Bianco A, Barbagallo M. The Importance of Vitamin D and Magnesium in Athletes. Nutrients. 2025 May 13;17(10):1655. doi: 10.3390/nu17101655.
- Background: PubMed 31443139Chavarrias M, Carlos-Vivas J, Collado-Mateo D, Perez-Gomez J. Health Benefits of Indoor Cycling: A Systematic Review. Medicina (Kaunas). 2019 Aug 8;55(8):452. doi: 10.3390/medicina55080452.
- Background: PubMed 18545183Battista RA, Foster C, Andrew J, Wright G, Lucia A, Porcari JP. Physiologic responses during indoor cycling. J Strength Cond Res. 2008 Jul;22(4):1236-41. doi: 10.1519/JSC.0b013e318173dbc4.
- Individual participant dataSharing planned: No. Individual participant data will not be shared because the dataset contains participant-level health and performance records. All results will be reported in aggregate form, and confidentiality will be maintained in accordance with applicable data protection regulations.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part.
Şemsi Gül Yılmaz Kocaman, PhD principal investigator
Karamanoğlu Mehmetbey University
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Spinning Training and Magnesium Supplementation in people with Oxidative Stress and Inflammation, run by Karamanoğlu Mehmetbey University at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 5 inclusion and 6 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: enrolling by invitation, as of the sponsor's last update on 23 Jun 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
This study is still under way. Results are not available in the source record yet.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Change in Maximal Oxygen Uptake (VO₂max) (time frame: Baseline and 6 weeks). The study lists 2 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
On the ClinicalTrials.gov record for NCT07662954, which also lists 7 linked publications.
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 23 Jun 2026.