Weight LossEX-RWL-LymphocNCT07567937
Effects of Exercise and Short Term Rapid Weight Loss on Whole Blood Cells and Lymphocyte Subsets in Highly Trained National Level Wrestlers
A study testing Exercise and Rapid Weight Loss in people with Weight Loss and Exercise, run by Ankara Yildirim Beyazıt University at 1 site in Turkey (Türkiye).
Trial summary
Wrestling is a weight-classified sport in which athletes frequently apply rapid weight loss (RWL) strategies prior to competition. While the performance-related consequences of RWL have been widely investigated, its combined effects with exercise on hematological parameters and immune cell profiles remain… Read more about this study
- Who is it for?
- Ages 18 to 35 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Weight Loss, Exercise
- What is being tested?
- Exercise and Rapid Weight Loss
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 9 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 21 Jun 2021to 10 Oct 2022
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 35 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Being between 18 and 35 years of age
- Being clinically healthy
- Actively competing in wrestling
- Having regular training experience
- No history of chronic disease
- No musculoskeletal injury that could affect performance
- Not using any medication or nutritional supplements that could influence hematological or biochemical parameters during the study period
- Willing to volunteer and provide written informed consent
Who cannot join
- Having any chronic disease (e.g., cardiovascular, metabolic, respiratory, or neurological disorders)
- Having a recent musculoskeletal injury that could affect exercise performance
- Using medications, ergogenic aids, or nutritional supplements that might influence hematological or biochemical parameters
- Having a history of smoking or alcohol abuse
- Failing to complete the testing procedures and training protocol required for the study
- Not providing written informed consent
- Withdrawing voluntarily during the study period
Trial locations and contacts
1 site in 1 country.
Turkey (Türkiye)1 site
Ankara Yildirim Beyazit University Sports Sciences Laboratory
Ankara, Ankara, 06000
About this study
Official title: Effects of Exercise and Short-Term Rapid Weight Loss on Whole Blood Cells and Lymphocyte Subsets in Highly Trained National-Level Wrestlers: A Two-Phase Repeated-Measures Study
Wrestling is a weight-classified sport in which athletes frequently apply rapid weight loss (RWL) strategies prior to competition. While the performance-related consequences of RWL have been widely investigated, its combined effects with exercise on hematological parameters and immune cell profiles remain insufficiently understood. Therefore, this study aims to examine the effects of short-term weight loss and exercise on whole blood cell parameters and lymphocyte subsets in elite wrestlers.
Nine elite male wrestlers actively competing in the senior category (mean age: 22.90 ± 5.15 years; mean height: 1.75 m) voluntarily participated in this study. Athletes will achieve an average body mass reduction of 4.3% over a five-day period. Venous blood samples will be collected at 09:30 a.m. in a fasted state during two experimental phases: (1) Exercise-only phase, with blood sampling before and after exercise (BRWL1 and BRWL2); (2) Exercise plus weight loss phase, following the five-day weight loss period, with blood sampling before and after exercise (ARWL1 and ARWL2). Complete blood count parameters will be analyzed using an automated hematology analyzer, while lymphocyte subsets (CD3+, CD4+, CD8+, CD19+, CD16/56+) will be assessed by flow cytometry. Statistical analyses will be performed with a significance level set at p < 0.05.
Read the detailed description
See brief summary for study protocol details
- Conditions
- Weight Loss, Exercise
- Being tested
- Exercise, Rapid Weight Loss
- Keywords
- Complete Blood Count, Exercise, Flow Cytometry, Lymphocyte Subsets, Immune System, Rapid Weight Loss
- Medical subject headings
- Weight Loss, Motor Activity
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
ExerciseOther
Also called Behavioral
Standardized wrestling exercise session; pre- and post-exercise venous blood collected
Rapid Weight LossOther
5-day rapid weight loss protocol achieving average 4.3% body mass reduction
| Study group | Role | What they receive |
|---|---|---|
| Exercise | Other | Participants performed a standardized wrestling exercise session on the first day of testing. Venous blood samples were collected pre- and post-exercise (pre-exercise: BRWL1; post-exercise: BRWL2) to evaluate hematological and lymphocyte subset responses. Receives: Other: Exercise |
| Exercise + Rapid Weight Loss | Other | Participants underwent a 5-day rapid weight loss protocol prior to the exercise session, achieving an average body mass reduction of 4.3%. On the fifth day, the same standardized exercise protocol as Group 1 was performed, and pre- and post-exercise blood samples were collected (pre-exercise: ARWL1; post-exercise: ARWL2) to evaluate hematological and lymphocyte subset responses. Receives: Other: Exercise; Other: Rapid Weight Loss |
What is being measured
The primary outcome is the main question the study is designed to answer.
Change in Leukocyte Count
Change in total white blood cell (WBC) count measured from pre- to post-exercise
Measured: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Change in Lymphocyte Percentage
Change in percentage of lymphocytes relative to total WBC count, measured from pre- to post-exercise.
Measured: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Change in CD3+ T Cell Count
Change in absolute count of CD3+ T lymphocytes measured from pre- to post-exercise.
Measured: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Change in CD4+ T Cell Count
Change in absolute count of CD4+ helper T lymphocytes measured from pre- to post-exercise.
Measured: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Change in CD8+ T Cell Count
Change in absolute count of CD8+ cytotoxic T lymphocytes measured from pre- to post-exercise.
Measured: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Change in CD19+ B Cell Count
Change in absolute count of CD19+ B lymphocytes measured from pre- to post-exercise.
Measured: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Change in CD16/56+ NK Cell Count
Change in absolute count of CD16/56+ natural killer (NK) cells measured from pre- to post-exercise.
Measured: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Secondary outcomes (1)
Change in Creatine Kinase and Lactic Acid Levels
Serum creatine kinase (CK) and lactic acid (LA) levels measured to assess anaerobic performance and muscle damage.
Measured: Pre-exercise and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted5 May 2026
- Study start21 Jun 2021
- Primary completion3 Apr 2022
- Study completion10 Oct 2022
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Weight LossStudies of the same condition, open and under way.
- More trials run by Ankara Yildirim Beyazıt UniversityThe sponsor's other registered studies.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Non-randomised
- Intervention model
- Sequential
- Masking
- None (open label)
- Enrolment
- 9 participants (actual)
Sponsor and investigators
- Lead sponsor
- Ankara Yildirim Beyazıt University
- Sponsor type
- University, hospital or other
- Responsible party
- Sponsor
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07567937
- Sponsor's protocol ID
- 2280
- Acronym
- EX-RWL-Lymphoc
- Status last verified
- May 2026
- First submitted
- 22 Apr 2026
- Last updated
- 15 May 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No
Study at a glance
From the registry record, last updated by the sponsor on 15 May 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Effects of Exercise and Short-Term Rapid Weight Loss on Whole Blood Cells and Lymphocyte Subsets in Highly Trained National-Level Wrestlers: A Two-Phase Repeated-Measures Study
- ClinicalTrials.gov ID
- NCT07567937
- Acronym / sponsor ID
- EX-RWL-Lymphoc / 2280
- Conditions
- Weight Loss, Exercise
- Keywords
- Complete Blood Count, Exercise, Flow Cytometry, Lymphocyte Subsets, Immune System, Rapid Weight Loss
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 9 participants (actual)
- Lead sponsor
- Ankara Yildirim Beyazıt UniversityUniversity, hospital or other
- Study start
- 21 Jun 2021 (actual)
- Primary completion
- 3 Apr 2022 (actual)
- Study completion
- 10 Oct 2022 (actual)
- First posted
- 5 May 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Wrestling is a weight-classified sport in which athletes frequently apply rapid weight loss (RWL) strategies prior to competition. While the performance-related consequences of RWL have been widely investigated, its combined effects with exercise on hematological parameters and immune cell profiles remain insufficiently understood. Therefore, this study aims to examine the effects of short-term weight loss and exercise on whole blood cell parameters and lymphocyte subsets in elite wrestlers.
Nine elite male wrestlers actively competing in the senior category (mean age: 22.90 ± 5.15 years; mean height: 1.75 m) voluntarily participated in this study. Athletes will achieve an average body mass reduction of 4.3% over a five-day period. Venous blood samples will be collected at 09:30 a.m. in a fasted state during two experimental phases: (1) Exercise-only phase, with blood sampling before and after exercise (BRWL1 and BRWL2); (2) Exercise plus weight loss phase, following the five-day weight loss period, with blood sampling before and after exercise (ARWL1 and ARWL2). Complete blood count parameters will be analyzed using an automated hematology analyzer, while lymphocyte subsets (CD3+, CD4+, CD8+, CD19+, CD16/56+) will be assessed by flow cytometry. Statistical analyses will be performed with a significance level set at p < 0.05.
Detailed description
See brief summary for study protocol details
Study design
- Primary purpose
- Basic science
- Allocation
- Non-randomised
- Intervention model
- Sequential
- Masking
- None (open label)
- Number of arms
- 2
| Arm | Type | Receives |
|---|---|---|
| Exercise | Other | Other: ExerciseParticipants performed a standardized wrestling exercise session on the first day of testing. Venous blood samples were collected pre- and post-exercise (pre-exercise: BRWL1; post-exercise: BRWL2) to evaluate hematological and lymphocyte subset responses. |
| Exercise + Rapid Weight Loss | Other | Other: Exercise, Other: Rapid Weight LossParticipants underwent a 5-day rapid weight loss protocol prior to the exercise session, achieving an average body mass reduction of 4.3%. On the fifth day, the same standardized exercise protocol as Group 1 was performed, and pre- and post-exercise blood samples were collected (pre-exercise: ARWL1; post-exercise: ARWL2) to evaluate hematological and lymphocyte subset responses. |
Who may qualify
- Ages
- 18 to 35 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (8 listed)
- Being between 18 and 35 years of age
- Being clinically healthy
- Actively competing in wrestling
- Having regular training experience
- No history of chronic disease
Key exclusion criteria (7 listed)
- Having any chronic disease (e.g., cardiovascular, metabolic, respiratory, or neurological disorders)
- Having a recent musculoskeletal injury that could affect exercise performance
- Using medications, ergogenic aids, or nutritional supplements that might influence hematological or biochemical parameters
- Having a history of smoking or alcohol abuse
- Failing to complete the testing procedures and training protocol required for the study
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| ExerciseAlso: BehavioralStandardized wrestling exercise session; pre- and post-exercise venous blood collected | Other | Exercise, Exercise + Rapid Weight Loss |
| Rapid Weight Loss5-day rapid weight loss protocol achieving average 4.3% body mass reduction | Other | Exercise + Rapid Weight Loss |
Primary outcome
Change in Leukocyte Count
Time frame: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Change in total white blood cell (WBC) count measured from pre- to post-exercise
Change in Lymphocyte Percentage
Time frame: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Change in percentage of lymphocytes relative to total WBC count, measured from pre- to post-exercise.
Change in CD3+ T Cell Count
Time frame: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Change in absolute count of CD3+ T lymphocytes measured from pre- to post-exercise.
Change in CD4+ T Cell Count
Time frame: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Change in absolute count of CD4+ helper T lymphocytes measured from pre- to post-exercise.
Change in CD8+ T Cell Count
Time frame: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Change in absolute count of CD8+ cytotoxic T lymphocytes measured from pre- to post-exercise.
Change in CD19+ B Cell Count
Time frame: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Change in absolute count of CD19+ B lymphocytes measured from pre- to post-exercise.
Change in CD16/56+ NK Cell Count
Time frame: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Change in absolute count of CD16/56+ natural killer (NK) cells measured from pre- to post-exercise.
1 secondary outcome
Change in Creatine Kinase and Lactic Acid Levels
Time frame: Pre-exercise and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 15 May 2026.
- Individual participant dataSharing planned: No
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Exercise and Rapid Weight Loss in people with Weight Loss and Exercise, run by Ankara Yildirim Beyazıt University at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 8 inclusion and 7 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 15 May 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Change in Leukocyte Count (time frame: Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group)). The study lists 7 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 15 May 2026.