Camel Milk Impact on Aerobic Exercise Recovery, Induced Inflammation, and Physical Performance

Study title Camel Milk Impact on Aerobic Exercise Recovery, Induced Inflammation, and Physical Performance

Investigator Dr. Mo'ath Bataineh Department of Nutrition and Health College of Medicine \& Health Sciences United Arab Emirates University Detailed Description of the Study and Procedures

1. Study Overview The research project, titled "Camel Milk Impact on Aerobic Exercise Recovery, Induced Inflammation, and Physical Performance," examines the physiological effects of post exercise camel milk consumption on high intensity cycling performance in recreationally trained adult males. The study was issued and approved on March 6, 2024, by the UAEU Human Research Ethics Committee. The investigation is conducted by Dr. Mo'ath Bataineh, Amjad Jarrar, and Zein Najjar from the Nutrition and Health Department, College of Medicine \& Health Sciences, United Arab Emirates University.
2. Purpose of the Study The primary objective is to determine whether consuming camel milk after strenuous exercise improves subsequent high intensity cycling performance. The secondary objectives include assessing the effects of camel milk on:

• Muscle damage
• Muscle soreness
• Inflammatory markers (IL 6, TNF α, IL 10)
• Time to exhaustion and workload capacity The central hypothesis is that camel milk consumption during recovery will:
• Enhance time to exhaustion and workload, and
• Attenuate increases in pro inflammatory markers, more effectively than cow's milk or water.

1. Participant Eligibility Participants are selected based on the following criteria:

• Male, non obese, over 18 years old
• No milk allergy
• Not consuming antioxidant or anti inflammatory supplements
• Not taking anti inflammatory drugs or antibiotics A total of 12 participants are enrolled.

1. Participant Rights and Voluntary Participation Participation is strictly voluntary. Individuals may withdraw at any time without providing a reason. A participant information sheet and consent form are provided.
2. Study Procedures 5.1 Number of Visits

Participants complete four laboratory visits:

Visit 1 - Training \& Baseline Measurements

• Educational workshop on accurate food and exercise logging
• Design of individualized standardized diet including 2 daily servings of dairy
• Measurements collected:
• Height and weight
• Peak work rate
• Heart rate
• Maximum speed at exhaustion
• VO₂MAX Visits 2-4 - Testing Days

Visits occur 4-5 days apart. Each visit includes:

1. Baseline measurements: resting heart rate, blood pressure, oxygen consumption, and CO₂ production
2. First cycling exercise on a bicycle ergometer
3. Recovery period:

• Immediate consumption of the assigned recovery beverage (camel milk, cow's milk, or water)
• 210 minute rest period

1. Second cycling exercise
2. Blood sampling at four time points: pre exercise, mid exercise, end of exercise, and during recovery
3. Measurement Protocols 6.1 Anthropometric Measurements

• Height: Measured on a digital column scale following standardized positioning guidelines.
• Body Composition: Measured using the InBody 720. Participants refrain from food/drink for 3 hours and empty bladder 30 minutes before testing. 6.2 Exercise Performance Testing
• Peak Power Output (PPO): Assessed using a cycle ergometer with warming-up procedures and gradual resistance increase.
• Heart Rate: Measured using the Polar H10 sensor.
• VO₂MAX: Determined using a calibrated metabolic cart (21-23 °C; reference gases 16% O₂, 3.99% CO₂).

1. Biological Sampling and Laboratory Analyses 7.1 Blood Sampling Performed by a licensed technician. Steps include vein identification, sterile needle insertion, sample labeling, and proper storage at 4-8 °C for ≤24 hours. 7.2 Plasma Collection Blood is centrifuged at 3200-3500 rpm for 10-15 minutes. Plasma is stored at -80 °C. 7.3 Hemoglobin \& Hematocrit Measured using HemoCue following standardized finger prick and cuvette handling procedures. 7.4 Creatine Kinase (CK) Assayed using the cobas c111 analyzer with reagent loading, calibration, QC checks, and automated calculation. 7.5 Cytokines

• IL 6: Measured using cobas e411 with reagent preparation, calibration, and QC.
• TNF α and IL 10: Assessed using ELISA with standard preparation, incubation, washing, TMB development, and absorbance reading at 450 nm.

1. Risks and Benefits There are no physical risks expected from participation. Participants may skip any question they find uncomfortable. While there may be no direct benefits, the study contributes to improved understanding of exercise recovery strategies.
2. Confidentiality All collected data remain strictly confidential:

• No names are recorded on forms.
• Data are accessible only to the research team.
• Electronic files are stored on password protected devices.
• Consent forms are stored securely in the PI's office.
• Publications and reports use group-level anonymized data only.

1. Dissemination of Results Results will be:

• Published in international peer reviewed journals
• Presented at scientific conferences
• Reported to the UAEU Human Research Ethics Committee and UAEU Research Office No individually identifiable data will be shared.

1. Funding and Oversight The study is organized by United Arab Emirates University and funded by the UAEU Research Office. Ethical oversight is provided by the UAEU Human Research Ethics Committee on February 7, 2024, (ERH_2023_3935).