Hair AgingNCT07123636
A Clinical Study to Assess the Effect of Hair Care Products on Grey Hair and Overall Hair Health
A study testing Hair Care Products in people with Hair Aging and Hair Graying, run by Two Lines LLC at 1 site in United States.
Trial summary
This is a 24-week virtual, single-arm study designed to evaluate the effectiveness of a hair care system-comprising the "Not Today, Grey" supplement, "To the Root" scalp serum, "Wash" shampoo, and "Smooth" conditioner-on grey hair reduction and overall hair health. Read more about this study
- Who is it for?
- Ages 25 to 55 monthsHealthy volunteers can take part
- Conditions the study is for
- Hair Aging, Hair Graying
- What is being tested?
- Hair Care Products
- Type of treatment
- Pill or oral medicine
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 70 participants
- Where
- 1 siteUnited States
- Study dates
- 1 Nov 2023to 30 Sep 2024
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 25 to 55 months
- All sexes
- Healthy volunteers accepted
- Child
Who may be able to join
- Male or female (35% male and 65% female)
- Aged 25-55
- Must experience 5-25% of hair being grey as confirmed by a dermatologist grading of the baseline photo (see Figure 1 below)
- Any hair color that the investigator can clearly distinguish grey hairs from the natural hair color.
- Individuals who have not dyed or highlighted their hair for at least 6 months prior to screening/baseline and who agree to refrain from dyeing the hair until after the final study visit.
- Willing to maintain the same hairstyle and length.
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- Willing to refrain from using any topical hair products (other than Wash, Smooth, and To The Root) or treatments on the hair that could affect hair properties (e.g., heat treatments other than blowdrying, medicated shampoo) for the duration of the study.
- Willing to refrain from taking any vitamins, minerals, or herbal supplements with claims related to hair growth or benefits during the study and to not start taking any new vitamins, minerals, or supplements of any kind during the study.
- Willing to comply with the protocol, take all required photos, and immediately report any changes in health status or medications, adverse events, symptoms, or reactions.
- Willing and able to provide written informed consent, including an agreement to privacy language compliant with country and/or local requirements, after the scope and nature of the investigation have been explained and before the initiation of any study-related procedures.
- Must have a recent smartphone (iPhone 11 pro or greater/android equivalent).
Who cannot join
- Anyone with pre-existing conditions that would prevent participants from adhering to the protocol, including oncological, immunological, psychiatric disorders, uncontrolled diseases, or multiple controlled diseases.
- Anyone pregnant, trying to conceive, or breastfeeding.
- Anyone unwilling to follow the protocol.
- Anyone currently participating in another research study.
- Anyone with any known severe allergic reactions or with any allergies of any severity to any of the test product's ingredients (e.g., sesame).
- Anyone with a medical history of any condition that results in grey hair (for example, nutritional deficiencies, such as B12, iron, and copper deficiency, severe protein malnutrition, cystic fibrosis, celiac disease, hyperthyroidism/hypothyroidism, vitiligo, alopecia areata, and genetic diseases such as Werner syndrome, Louis-Bar syndrome, Waardenburg syndrome, or Griscelli syndrome).
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- Having a medical history of any condition that results in hair darkening (e.g., Addison's disease, neurodermatitis, porphyria cutanea tarda, and inflammatory scalp conditions).
- Anyone currently receiving iron injections.
- Anyone who is currently a smoker or has smoked regularly in the last six months.
- Anyone who has a history of substance abuse (i.e., within six months prior to screening).
- Anyone who has started long-term medication within the last two months.
- Anyone who is having any planned surgeries or invasive procedures during the study period (24 weeks) or has undergone an invasive procedure three weeks prior to the study start.
- Anyone who has used an investigational drug or device within the 30 days prior to screening.
- Anyone who is currently undergoing hormone therapy (e.g., HRT, or testosterone) and anyone who in the last three months has undergone hormone therapy.
Trial locations and contacts
1 site in 1 country.
About this study
This is a 24-week virtual, single-arm study designed to evaluate the effectiveness of a hair care system-comprising the "Not Today, Grey" supplement, "To the Root" scalp serum, "Wash" shampoo, and "Smooth" conditioner-on grey hair reduction and overall hair health. Assessments include clinical grading of photographic evidence by dermatologists and participant-reported outcomes.
- Conditions
- Hair Aging, Hair Graying
- Being tested
- Hair Care Products
- Keywords
- Hair Health, Hair Aging
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Hair Care ProductsDietary supplement
Daily oral intake of 2 tablets (Not Today, Grey). Twice-daily application of 3-4 pumps of "To The Root" serum. Wash and Smooth used according to hair type (daily or every other day).
| Study group | Role | What they receive |
|---|---|---|
| Hair Care Products | Experimental | All participants will receive the four products and use them as directed over 24 weeks. Receives: Dietary Supplement: Hair Care Products |
What is being measured
The primary outcome is the main question the study is designed to answer.
Change in Grey Hair Assessed by Dermatologists
Assessment of photographic evidence (baseline, week 12, and week 24) to evaluate reduction in grey hair. Two trained dermatologists will review photos in a blinded order and answer yes/no questions on greying reduction and repigmentation.
Measured: Baseline, Week 12, Week 24
Secondary outcomes (1)
Participant Self-Reported Changes in Hair Health
Participants will complete questionnaires assessing their perception of grey hair amount, hair softness, shine, texture, strength, thickness, and overall hair quality.
Measured: Weeks 6, 12, 18, and 24
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted14 Aug 2025
- Study start1 Nov 2023
- Primary completion30 Sep 2024
- Study completion30 Sep 2024
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Hair AgingStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Treatment
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Enrolment
- 70 participants (actual)
Sponsor and investigators
- Lead sponsor
- Two Lines LLC
- Sponsor type
- Industry (pharma, biotech, devices)
- Collaborators
- Citruslabs
- Responsible party
- Sponsor
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07123636
- Sponsor's protocol ID
- 20385
- Status last verified
- Aug 2025
- First submitted
- 22 Apr 2025
- Last updated
- 14 Aug 2025
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No
Study at a glance
From the registry record, last updated by the sponsor on 14 Aug 2025. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- A Clinical Study to Assess the Effect of Hair Care Products on Grey Hair and Overall Hair Health
- ClinicalTrials.gov ID
- NCT07123636
- Acronym / sponsor ID
- 20385
- Conditions
- Hair Aging, Hair Graying
- Keywords
- Hair Health, Hair Aging
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 70 participants (actual)
- Lead sponsor
- Two Lines LLCIndustry (pharma, biotech, devices)
- Collaborators
- Citruslabs
- Study start
- 1 Nov 2023 (actual)
- Primary completion
- 30 Sep 2024 (actual)
- Study completion
- 30 Sep 2024 (actual)
- First posted
- 14 Aug 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
This is a 24-week virtual, single-arm study designed to evaluate the effectiveness of a hair care system-comprising the "Not Today, Grey" supplement, "To the Root" scalp serum, "Wash" shampoo, and "Smooth" conditioner-on grey hair reduction and overall hair health. Assessments include clinical grading of photographic evidence by dermatologists and participant-reported outcomes.
Study design
- Primary purpose
- Treatment
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Number of arms
- 1
| Arm | Type | Receives |
|---|---|---|
| Hair Care Products | Experimental | Dietary Supplement: Hair Care ProductsAll participants will receive the four products and use them as directed over 24 weeks. |
Who may qualify
- Ages
- 25 to 55 months
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Child
Key inclusion criteria (11 listed)
- Male or female (35% male and 65% female)
- Aged 25-55
- Must experience 5-25% of hair being grey as confirmed by a dermatologist grading of the baseline photo (see Figure 1 below)
- Any hair color that the investigator can clearly distinguish grey hairs from the natural hair color.
- Individuals who have not dyed or highlighted their hair for at least 6 months prior to screening/baseline and who agree to refrain from dyeing the hair until after the final study visit.
Key exclusion criteria (14 listed)
- Anyone with pre-existing conditions that would prevent participants from adhering to the protocol, including oncological, immunological, psychiatric disorders, uncontrolled diseases, or multiple controlled diseases.
- Anyone pregnant, trying to conceive, or breastfeeding.
- Anyone unwilling to follow the protocol.
- Anyone currently participating in another research study.
- Anyone with any known severe allergic reactions or with any allergies of any severity to any of the test product's ingredients (e.g., sesame).
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Hair Care ProductsDaily oral intake of 2 tablets (Not Today, Grey). Twice-daily application of 3-4 pumps of "To The Root" serum. Wash and Smooth used according to hair type (daily or every other day). | Dietary supplement | Hair Care Products |
Primary outcome
Change in Grey Hair Assessed by Dermatologists
Time frame: Baseline, Week 12, Week 24
Assessment of photographic evidence (baseline, week 12, and week 24) to evaluate reduction in grey hair. Two trained dermatologists will review photos in a blinded order and answer yes/no questions on greying reduction and repigmentation.
1 secondary outcome
Participant Self-Reported Changes in Hair Health
Time frame: Weeks 6, 12, 18, and 24
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 14 Aug 2025.
- Individual participant dataSharing planned: No
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Hair Care Products in people with Hair Aging and Hair Graying, run by Two Lines LLC at 1 site in United States.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 11 inclusion and 14 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 14 Aug 2025. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in United States. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Change in Grey Hair Assessed by Dermatologists (time frame: Baseline, Week 12, Week 24). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 14 Aug 2025.