Ramadan FastingNCT06767176
Impact of Dietary Nitrate Supplementation on VO2max During Ramadan Fasting in Healthy Adults
A study testing Beetroot Juice (Beet-It Stamina Shot) & Supervised Exercise Training in people with Ramadan Fasting, run by University of Guelph at 1 site in Canada.
Trial summary
The goal of this clinical trial is to determine if beetroot juice supplementation changes exercise performance following one week of Ramadan fasting in young, healthy adults. The main question it aims to answer is: Does daily beetroot juice supplementation change VO2 max in fasting individuals during the first week of… Read more about this study
- Who is it for?
- Ages 18 to 50 years
- Conditions the study is for
- Ramadan Fasting
- What is being tested?
- Beetroot Juice (Beet-It Stamina Shot) & Supervised Exercise Training
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 21 participants
- Where
- 1 siteCanada
- Study dates
- 6 Jan 2025to 31 Mar 2025
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 50 years
- All sexes
- No healthy volunteers
- Adult
Who may be able to join
- Free of known cardiovascular or metabolic diseases or sleep disorders
- No history of smoking (within the past 3 months)
- Able to engage in physical activity assessed through the physical activity readiness questionnaire (PAR-Q+)
- No prescription of chronic medications other than oral contraceptives
- Able to abide by fasting protocols for all visits
- Individuals who are not allergic to ultrasound gel or beetroot juice
- Individuals who are not pregnant
Who cannot join
- Individuals diagnosed with cardiovascular or metabolic disease or sleep disorders
- Has a history of smoking (within the past 3 months)
- Not ready to engage in physical activity as assessed by the PAR-Q+
- Individuals prescribed chronic medications other than oral contraceptives
- Unable to abide by fasting protocols for any testing visit
- Allergic to ultrasound gel or beetroot juice
- Pregnancy
Trial locations and contacts
1 site in 1 country.
About this study
The goal of this clinical trial is to determine if beetroot juice supplementation changes exercise performance following one week of Ramadan fasting in young, healthy adults. The main question it aims to answer is:
Does daily beetroot juice supplementation change VO2 max in fasting individuals during the first week of Ramadan?
Participants will:
Visit the lab before the start of Ramadan and after one week of fasting If assigned to intervention group, consume daily shots of beetroot juice (70ml) during the first week of Ramadan fasting Perform maximal exercise testing on a cycle ergometer
Read the detailed description
Recent literature demonstrates data showing a decrease in VO2max during the first week of Ramadan fasting. This is a practice observed by millions around the world every year, where individuals abstain from food and water from sunrise to sunset, dictated by the lunar calendar. Dietary nitrate consumption has been shown to increase VO2max and exercise performance. Therefore, the primary purpose of this study is to investigate whether dietary nitrate, in the form of beetroot juice, can help mitigate the decrease in VO2 max during the first week of Ramadan fasting.
24 young healthy adults will be recruited to undergo these two visits. The first visit will assess maximal oxygen uptake to determine aerobic fitness and metabolic exercise intensity zones under non-fasting conditions. The second visit will be completed after one week of Ramadan fasting. If assigned to the intervention group, participants will consume one shot (70ml) of Blonyx - Beet It Sport Nitrate 400 beetroot juice every day. Each shot contains 400mg of nitrates. Urine Specific Gravity will be measured after each visit to assess hydration status. The intervention group will also undergo Single Blood Draws after each visit to assess nitrate concentrations before and after a week of consumption.
- Conditions
- Ramadan Fasting
- Being tested
- Beetroot Juice (Beet-It Stamina Shot) & Supervised Exercise Training
- Keywords
- Ramadan, Fasting, VO2max, Dietary Nitrate
- Medical subject headings
- Fasting
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Beetroot Juice (Beet-It Stamina Shot) & Supervised Exercise TrainingDietary supplement
7 doses, taken daily, 70 ml shots, consumed in the morning before the start of fasting
| Study group | Role | What they receive |
|---|---|---|
| No Intervention | No intervention | Normal Fasting, no intervention. During this arm, participants will be asked to complete an exercise test before and after 1 week of Ramadan fasting. |
| Nitrate Supplementation | Experimental | Participants will be asked to consume daily nitrate supplementation (70ml) in the form of beetroot juice for 7 days. Participants will then complete a maximal exercise test as they fast. Receives: Dietary Supplement: Beetroot Juice (Beet-It Stamina Shot) & Supervised Exercise Training |
What is being measured
The primary outcome is the main question the study is designed to answer.
VO2 max
Maximal Oxygen Uptakes will be assessed to analyze aerobic capacity and fitness under fasting conditions.
Measured: One--week differences between before and after 1 week of fasting, nitrate supplementation if in intervention group
Secondary outcomes (1)
Nitrate Concentration levels
Single blood draws will be taken after each visit for the nitrate supplementation group
Measured: Comparison between blood before and after nitrate supplementation: one week
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted9 Jan 2025
- Study start6 Jan 2025
- Primary completion31 Mar 2025
- Study completion31 Mar 2025
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Ramadan FastingStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- None (open label)
- Enrolment
- 21 participants (actual)
Sponsor and investigators
- Lead sponsor
- University of Guelph
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Philip Millar, University of Guelph
- Investigators
- Phillip Millar, PhD, Principal investigator, University of Guelph
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT06767176
- Sponsor's protocol ID
- 25-01-006
- Status last verified
- Apr 2026
- First submitted
- 6 Jan 2025
- Last updated
- 1 May 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- Yes: IPD used in the results publication
Study at a glance
From the registry record, last updated by the sponsor on 1 May 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Impact of Dietary Nitrate Supplementation on VO2max During Ramadan Fasting in Healthy Adults
- ClinicalTrials.gov ID
- NCT06767176
- Acronym / sponsor ID
- 25-01-006
- Conditions
- Ramadan Fasting
- Keywords
- Ramadan, Fasting, VO2max, Dietary Nitrate
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 21 participants (actual)
- Lead sponsor
- University of GuelphUniversity, hospital or other
- Study start
- 6 Jan 2025 (actual)
- Primary completion
- 31 Mar 2025 (actual)
- Study completion
- 31 Mar 2025 (actual)
- First posted
- 9 Jan 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The goal of this clinical trial is to determine if beetroot juice supplementation changes exercise performance following one week of Ramadan fasting in young, healthy adults. The main question it aims to answer is:
Does daily beetroot juice supplementation change VO2 max in fasting individuals during the first week of Ramadan?
Participants will:
Visit the lab before the start of Ramadan and after one week of fasting If assigned to intervention group, consume daily shots of beetroot juice (70ml) during the first week of Ramadan fasting Perform maximal exercise testing on a cycle ergometer
Detailed description
Recent literature demonstrates data showing a decrease in VO2max during the first week of Ramadan fasting. This is a practice observed by millions around the world every year, where individuals abstain from food and water from sunrise to sunset, dictated by the lunar calendar. Dietary nitrate consumption has been shown to increase VO2max and exercise performance. Therefore, the primary purpose of this study is to investigate whether dietary nitrate, in the form of beetroot juice, can help mitigate the decrease in VO2 max during the first week of Ramadan fasting.
24 young healthy adults will be recruited to undergo these two visits. The first visit will assess maximal oxygen uptake to determine aerobic fitness and metabolic exercise intensity zones under non-fasting conditions. The second visit will be completed after one week of Ramadan fasting. If assigned to the intervention group, participants will consume one shot (70ml) of Blonyx - Beet It Sport Nitrate 400 beetroot juice every day. Each shot contains 400mg of nitrates. Urine Specific Gravity will be measured after each visit to assess hydration status. The intervention group will also undergo Single Blood Draws after each visit to assess nitrate concentrations before and after a week of consumption.
Study design
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- None (open label)
- Number of arms
- 2
| Arm | Type | Receives |
|---|---|---|
| No Intervention | No intervention | –Normal Fasting, no intervention. During this arm, participants will be asked to complete an exercise test before and after 1 week of Ramadan fasting. |
| Nitrate Supplementation | Experimental | Dietary Supplement: Beetroot Juice (Beet-It Stamina Shot) & Supervised Exercise TrainingParticipants will be asked to consume daily nitrate supplementation (70ml) in the form of beetroot juice for 7 days. Participants will then complete a maximal exercise test as they fast. |
Who may qualify
- Ages
- 18 to 50 years
- Sex
- All
- Healthy volunteers
- Not accepted
- Age groups
- Adult
Key inclusion criteria (7 listed)
- Free of known cardiovascular or metabolic diseases or sleep disorders
- No history of smoking (within the past 3 months)
- Able to engage in physical activity assessed through the physical activity readiness questionnaire (PAR-Q+)
- No prescription of chronic medications other than oral contraceptives
- Able to abide by fasting protocols for all visits
Key exclusion criteria (7 listed)
- Individuals diagnosed with cardiovascular or metabolic disease or sleep disorders
- Has a history of smoking (within the past 3 months)
- Not ready to engage in physical activity as assessed by the PAR-Q+
- Individuals prescribed chronic medications other than oral contraceptives
- Unable to abide by fasting protocols for any testing visit
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Beetroot Juice (Beet-It Stamina Shot) & Supervised Exercise Training7 doses, taken daily, 70 ml shots, consumed in the morning before the start of fasting | Dietary supplement | Nitrate Supplementation |
Primary outcome
VO2 max
Time frame: One--week differences between before and after 1 week of fasting, nitrate supplementation if in intervention group
Maximal Oxygen Uptakes will be assessed to analyze aerobic capacity and fitness under fasting conditions.
1 secondary outcome
Nitrate Concentration levels
Time frame: Comparison between blood before and after nitrate supplementation: one week
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 1 May 2026.
- Intervention Wesbite.Link provided by the sponsor.
- Individual participant dataSharing planned: Yes. IPD used in the results publication
Related trials for Ramadan Fasting
Active, not recruitingEarly Phase 1
Optimization of Basal Insulin During Ramadan Fasting in Type 2 Diabetic Patients: Comparison Between DAR Recommendations and the Carbohydrate Fasting Test & Metabolic and Inflammatory Consequences
Diabetes Mellitus, Type 2 Treated With Insulin, Fasting, Intermittent
Not yet recruiting
Risks of Ramadan Fasting in Patients With Primary Adrenal Insufficiency Treated With Prednisolone.
Primary Adrenal Insufficiency, Ramadan Fasting
Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
Phillip Millar, PhD principal investigator
University of Guelph
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Beetroot Juice (Beet-It Stamina Shot) & Supervised Exercise Training in people with Ramadan Fasting, run by University of Guelph at 1 site in Canada.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 7 inclusion and 7 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 1 May 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Canada. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
VO2 max (time frame: One--week differences between before and after 1 week of fasting, nitrate supplementation if in intervention group). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 1 May 2026; AzTrials last refreshed its copy on 7 Nov 2026.