Dietary SupplementsCAF+BJNCT06596395
Effect of Acute Caffeine and Beetroot Juice Intake on Strength, Power, and Muscular Endurance
A study testing Acute caffeine and beetroot juice, Acute caffeine and Acute beetroot juice in people with Dietary Supplements and Caffeine, run by University of Alcala at 1 site in Spain.
Trial summary
Introduction: Several studies have evaluated and confirmed the ergogenic effect of acute caffeine and beetroot juice intake on sports performance. However, there are few studies focused on the production of strength, power, and muscle endurance, particularly when both supplements are ingested together. Read more about this study
- Who is it for?
- Ages 18 to 35 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Dietary Supplements, Caffeine, Nitrates
- What is being tested?
- Acute caffeine and beetroot juice, Acute caffeine and Acute beetroot juice
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 14 participants
- Where
- 1 siteSpain
- Study dates
- 1 Sep 2023to 1 May 2024
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 35 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Age between ≥ 18 and ≤ 35 years.
- Body Mass Index (BMI) \< 25 kg/m².
- Resistance-trained individuals (\> 2 years of structured trained).
- Healthy men and women without neurological, cardiometabolic, immunological, or physical conditions that prevent them from exercising.
- Participants must be able to perform the tests described in the following section.
Who cannot join
- History of neuromuscular diseases, heart diseases, or diseases that may affect liver or muscle metabolism.
- Use of drugs, other stimulants or sport supplements that interfere with the intake of the study\'s dietary supplements.
- Sedentary habits (\< 150 min/week of moderate exercise).
- Having undergone prolonged periods of forced physical inactivity during the 6 months prior to the study.
- Performing strenuous exercise during the 48 hours prior to the tests.
- Failure to replicate the same food intake on the two days of the experiments.
- Consuming caffeine or any other stimulant after 6 pm the day before the training or tests, to avoid headaches, discomfort, or lethargy among regular caffeine consumers.
- Consuming foods rich in NO3- (e.g., beetroot, spinach, or celery) during the 48 hours prior to each experimental condition.
Trial locations and contacts
1 site in 1 country.
Spain1 site
Facultad de Medicina y Ciencias de la Salud. Universidad de Alcalá
Alcalá de Henares, Madrid, 28805
About this study
Official title: Effect of Acute Caffeine and Beetroot Juice Intake on Strength, Power, and Muscular Endurance Based on Circadian Rhythms.
Introduction: Several studies have evaluated and confirmed the ergogenic effect of acute caffeine and beetroot juice intake on sports performance. However, there are few studies focused on the production of strength, power, and muscle endurance, particularly when both supplements are ingested together.
Objectives: Evaluate the acute effect of combined and isolated intake of caffeine and beetroot juice on the production of strength, power, and muscle endurance in young adults, analyzing the effect of circadian rhythms (morning vs. afternoon) on the exercise performed (bench press vs. squat).
Methods: A total of 12 healthy and physically active participants will be recruited for the study. Through a double-blind, crossover, randomized, and controlled design, participants will ingest: a) Caffeine (3 mg/kg body mass); b) Beetroot juice (70 ml, 6.5 mmol of nitrates); c) Caffeine + Beetroot juice; d) Placebo (maltodextrin, 3 mg/kg). The supplements will be ingested 120 minutes (beetroot juice or placebo) and 60 minutes before (caffeine or placebo), the latter dissolved in 200 ml of water. After the ingestion period, strength, power, and muscle endurance will be analyzed with 1RM tests, strength and power tests at 25%, 50%, 75%, and 90% 1RM, and muscle endurance tests at 65% 1RM, in all cases for squat and bench press exercises. Additionally, dynamometry (manual and the isometric mid-thigh pull test) and vertical jump (squat jump and countermovement jump) will be analyzed. The four experimental conditions will be conducted in the morning (8:00 am to 10:00 am) and in the afternoon (4:30 pm to 6:30 pm).
- Conditions
- Dietary Supplements, Caffeine, Nitrates, Sports Performance, Resistance Exercise
- Being tested
- Acute caffeine and beetroot juice, Acute caffeine, Acute beetroot juice, Placebo
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Acute caffeine and beetroot juiceDietary supplement
Combined effect of beetroot juice and caffeine intake on muscular strength, power and endurance performance
Acute caffeineDietary supplement
single effect of caffeine intake on muscular strength, power and endurance performance
Acute beetroot juiceDietary supplement
single effect of beetroot juice intake on muscular strength, power and endurance performance
PlaceboDietary supplement
single effect of placebo on muscular strength, power and endurance performance
| Study group | Role | What they receive |
|---|---|---|
| Caffeine + Beetroot Juice | Experimental | 70 mL of concentrated NO3--rich (BJ, 6.5 mmol NO3-) + 3 mg/kg of caffeine Receives: Dietary Supplement: Acute caffeine and beetroot juice |
| Beetroot Juice | Experimental | 70 mL of concentrated NO3--rich (BJ, 6.5 mmol NO3-) + 3 mg/kg of placebo (maltodextrin) Receives: Dietary Supplement: Acute beetroot juice |
| Caffeine | Experimental | 70 mL of NO3--depleted (BJ, 0.04 mmol NO3-) + 3 mg/kg of caffeine Receives: Dietary Supplement: Acute caffeine |
| Placebo | Placebo comparator | 70 mL of NO3--depleted (BJ, 0.04 mmol NO3-) + 3 mg/kg of placebo (maltodextrin) Receives: Dietary Supplement: Placebo |
What is being measured
The primary outcome is the main question the study is designed to answer.
Mean and peak velocity and time to reach peak velocity at different %1RM
Measuring bar velocity desplacement during bench press and back squat exercises.
Measured: On each trial, after dietary supplement intake, during 2 weeks
Mean and peak power output and time to reach peak power output at different %1RM
Measuring power output generated during bench press and back squat exercises.
Measured: On each trial, after dietary supplement intake, during 2 weeks
Number of repetitions performed at 65%1RM until task failure
Additionally to mean and peak velocity, mean and peak power output, time to reach peak velocity and peak power
Measured: On each trial, after dietary supplement intake, during 2 weeks
Secondary outcomes (4)
Mood state (tension, depression, anger, vigor, fatigue and confusion)
Participants graded a set of 29 items related to the mood on a Likert scale from 0 (not at all) to 4 (extremely) in reply to the question \"How do you feel at this moment?\" to assess six scales: tension, depression, anger, vigor, fatigue and confusion.
Measured: On each trial, after dietary supplement intake, during 2 weeks
Adverse effects
perception of power, endurance, energy and exertion, as well as heart, muscular and gastrointestinal discomfort.
Measured: On each trial, after dietary supplement intake, during 2 weeks
handgrip and isometric mid-thigh pull tests
N generated
Measured: On each trial, after dietary supplement intake, during 2 weeks
Vertical Jump (cm and power)
Measured: On each trial, after dietary supplement intake, during 2 weeks
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted19 Sep 2024
- Study start1 Sep 2023
- Primary completion30 Jan 2024
- Study completion1 May 2024
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Dietary SupplementsStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Model description
- Triple-blind, cross-over, randomized controlled-trial
- Masking
- Triple blind
- Who is masked
- Participant, Investigator, Outcomes assessor
- Enrolment
- 14 participants (actual)
Sponsor and investigators
- Lead sponsor
- University of Alcala
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Alberto Pérez-López, University of Alcala
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT06596395
- Sponsor's protocol ID
- CEIP/2023/4/093
- Acronym
- CAF+BJ
- Status last verified
- Sep 2024
- First submitted
- 6 Sep 2024
- Last updated
- 19 Sep 2024
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- Yes
Study at a glance
From the registry record, last updated by the sponsor on 19 Sep 2024. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Effect of Acute Caffeine and Beetroot Juice Intake on Strength, Power, and Muscular Endurance Based on Circadian Rhythms.
- ClinicalTrials.gov ID
- NCT06596395
- Acronym / sponsor ID
- CAF+BJ / CEIP/2023/4/093
- Conditions
- Dietary Supplements, Caffeine, Nitrates, Sports Performance, Resistance Exercise
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 14 participants (actual)
- Lead sponsor
- University of AlcalaUniversity, hospital or other
- Study start
- 1 Sep 2023 (actual)
- Primary completion
- 30 Jan 2024 (actual)
- Study completion
- 1 May 2024 (actual)
- First posted
- 19 Sep 2024 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Introduction: Several studies have evaluated and confirmed the ergogenic effect of acute caffeine and beetroot juice intake on sports performance. However, there are few studies focused on the production of strength, power, and muscle endurance, particularly when both supplements are ingested together.
Objectives: Evaluate the acute effect of combined and isolated intake of caffeine and beetroot juice on the production of strength, power, and muscle endurance in young adults, analyzing the effect of circadian rhythms (morning vs. afternoon) on the exercise performed (bench press vs. squat).
Methods: A total of 12 healthy and physically active participants will be recruited for the study. Through a double-blind, crossover, randomized, and controlled design, participants will ingest: a) Caffeine (3 mg/kg body mass); b) Beetroot juice (70 ml, 6.5 mmol of nitrates); c) Caffeine + Beetroot juice; d) Placebo (maltodextrin, 3 mg/kg). The supplements will be ingested 120 minutes (beetroot juice or placebo) and 60 minutes before (caffeine or placebo), the latter dissolved in 200 ml of water. After the ingestion period, strength, power, and muscle endurance will be analyzed with 1RM tests, strength and power tests at 25%, 50%, 75%, and 90% 1RM, and muscle endurance tests at 65% 1RM, in all cases for squat and bench press exercises. Additionally, dynamometry (manual and the isometric mid-thigh pull test) and vertical jump (squat jump and countermovement jump) will be analyzed. The four experimental conditions will be conducted in the morning (8:00 am to 10:00 am) and in the afternoon (4:30 pm to 6:30 pm).
Study design
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Triple blind (participant, investigator, outcomes assessor)
- Number of arms
- 4
- Model description
- Triple-blind, cross-over, randomized controlled-trial
| Arm | Type | Receives |
|---|---|---|
| Caffeine + Beetroot Juice | Experimental | Dietary Supplement: Acute caffeine and beetroot juice70 mL of concentrated NO3--rich (BJ, 6.5 mmol NO3-) + 3 mg/kg of caffeine |
| Beetroot Juice | Experimental | Dietary Supplement: Acute beetroot juice70 mL of concentrated NO3--rich (BJ, 6.5 mmol NO3-) + 3 mg/kg of placebo (maltodextrin) |
| Caffeine | Experimental | Dietary Supplement: Acute caffeine70 mL of NO3--depleted (BJ, 0.04 mmol NO3-) + 3 mg/kg of caffeine |
| Placebo | Placebo comparator | Dietary Supplement: Placebo70 mL of NO3--depleted (BJ, 0.04 mmol NO3-) + 3 mg/kg of placebo (maltodextrin) |
Who may qualify
- Ages
- 18 to 35 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (5 listed)
- Age between ≥ 18 and ≤ 35 years.
- Body Mass Index (BMI) \< 25 kg/m².
- Resistance-trained individuals (\> 2 years of structured trained).
- Healthy men and women without neurological, cardiometabolic, immunological, or physical conditions that prevent them from exercising.
- Participants must be able to perform the tests described in the following section.
Key exclusion criteria (8 listed)
- History of neuromuscular diseases, heart diseases, or diseases that may affect liver or muscle metabolism.
- Use of drugs, other stimulants or sport supplements that interfere with the intake of the study\'s dietary supplements.
- Sedentary habits (\< 150 min/week of moderate exercise).
- Having undergone prolonged periods of forced physical inactivity during the 6 months prior to the study.
- Performing strenuous exercise during the 48 hours prior to the tests.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Acute caffeine and beetroot juiceCombined effect of beetroot juice and caffeine intake on muscular strength, power and endurance performance | Dietary supplement | Caffeine + Beetroot Juice |
| Acute caffeinesingle effect of caffeine intake on muscular strength, power and endurance performance | Dietary supplement | Caffeine |
| Acute beetroot juicesingle effect of beetroot juice intake on muscular strength, power and endurance performance | Dietary supplement | Beetroot Juice |
| Placebosingle effect of placebo on muscular strength, power and endurance performance | Dietary supplement | Placebo |
Primary outcome
Mean and peak velocity and time to reach peak velocity at different %1RM
Time frame: On each trial, after dietary supplement intake, during 2 weeks
Measuring bar velocity desplacement during bench press and back squat exercises.
Mean and peak power output and time to reach peak power output at different %1RM
Time frame: On each trial, after dietary supplement intake, during 2 weeks
Measuring power output generated during bench press and back squat exercises.
Number of repetitions performed at 65%1RM until task failure
Time frame: On each trial, after dietary supplement intake, during 2 weeks
Additionally to mean and peak velocity, mean and peak power output, time to reach peak velocity and peak power
4 secondary outcomes
Mood state (tension, depression, anger, vigor, fatigue and confusion)
Time frame: On each trial, after dietary supplement intake, during 2 weeks
Adverse effects
Time frame: On each trial, after dietary supplement intake, during 2 weeks
handgrip and isometric mid-thigh pull tests
Time frame: On each trial, after dietary supplement intake, during 2 weeks
Vertical Jump (cm and power)
Time frame: On each trial, after dietary supplement intake, during 2 weeks
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 19 Sep 2024.
- Individual participant dataSharing planned: Yes
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Acute caffeine and beetroot juice, Acute caffeine and Acute beetroot juice in people with Dietary Supplements and Caffeine, run by University of Alcala at 1 site in Spain.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 5 inclusion and 8 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 19 Sep 2024. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Spain. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. Some participants in this study may receive a placebo. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Mean and peak velocity and time to reach peak velocity at different %1RM (time frame: On each trial, after dietary supplement intake, during 2 weeks). The study lists 3 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 19 Sep 2024.