Pulsed tVNS Protocol and Reinforcement Learning

The study follows a single-blind randomized cross-over design with only 1 within-subject factor (stimulation condition, 2 levels: taVNS, sham) leading to 2 experimental sessions per participant.

Previous studies mainly focused on administering tonic stimulation, applied continuously with a 30s ON 30s OFF stimulation protocol for the full task duration. More recently, protocols delivering short taVNS bursts have been developed to more closely mimic vagal signals. However, optimal parameters (e.g., stimulation length, frequency) to elicit phasic signals are not yet determined. To test which combination of parameters most successfully elicits phasic responses, The investigators conducted a pilot data collection with a set of parameters comparing taVNS and sham stimulation. As a proxy for phasic signaling, the investigators investigated pupil dilations mediated by the locus coeruleus, a main target of the vagus nerve. Ten participants were invited for 2 sessions (taVNS, sham) during which they received pulsed stimulation with the following settings: stimulation duration of 1, 2, or 3s, stimulation frequency of 20, 25, or 30Hz. As a result of this pilot, the investigators found that most pilot participants (7 out of 10) showed the strongest taVNS-induced response vs. sham when stimulation for 1s with 20 Hz frequency. Therefore, this combination of settings will be implemented during the main study. For stimulation, the tVNS Health - R® (tVNS Health, Erlangen, Germany) is used and for eye-tracking the SR Research Eyelink 1000Plus®.

For the main study, participants will be invited to three experimental sessions. The investigators observed considerable interindividual variability in the pupil response to stimulation and similar variability has been previously described for taVNS-induced brain responses. This variability might be partly related to different anatomical characteristics. Therefore, participants will first complete an eye-tracking session to evaluate their pupil response to short taVNS pulses. The investigators then only invite participants responding to taVNS to the neuroimaging sessions to test whether short stimulation pulses can induce phasic signals in principle. In the first session, informed consent is obtained. The pupil response to stimulation with the chosen stimulation settings (1s and 20Hz, 45 trials, ~25min) comparing taVNS and sham stimulation (two blocks separated by a 5-minute break following a randomized order) will be tested to confirm that they elicit a robust pupillary response. taVNS at the right cymba concha for active taVNS and sham stimulation at the earlobe will be used. For the two neuroimaging sessions, the investigators plan to invite participants who showed larger pupil dilation for taVNS vs. sham stimulation during the first session. The investigators will administer taVNS in one session and sham in the other. Session order will be randomized. During calibration, the stimulation intensity will be adjusted to correspond to a mild pricking sensation for taVNS and sham. As previously reported, the intensity will be increased from 0.1 mA in 0.1 mA increments until the participant reports experiencing this sensation. To ensure effective blinding, the investigators will pair non-winning cues (for the blocks in which stimulation is paired with rewarding feedback) or actions associated with the incorrect stimulus (the cue less likely to give a reward in the blocks where stimulation is paired with an action) with a low-intensity (0.1 mA) stimulation. This serves as an additional control condition to compare the effects of the actual stimulation (high intensity) with stimulation that should not activate vagal afferents (low intensity). Participants will be informed that they will receive stimulation with varying intensity in all trials, even though the stimulation won't always be perceived.

Before entering the scanner, participants will complete a short practice of the instrumental learning task to become familiarized with the structure and procedure of the task. Then, participants will enter the fMRI scanner. Here the investigators will conduct a calibration of the stimulation intensity as well as another short practice of the task to calibrate the grip force device used during the task. After this second practice, an anatomical image will be acquired, followed by a resting-state measurement for functional connectivity analyses. During the measurement, a movie will be shown to reduce movement and prevent the participant from falling asleep (Inscapes). To conclude the session, participants will perform the instrumental learning task (described in the section below) with concurrent taVNS or sham stimulation (~1h of stimulation).

Instrumental Learning Task A probabilistic learning task to evaluate the potential of pulsed taVNS on instrumental learning will be used where participants must learn which actions are related to higher probabilities of reward based on specific cues. Participants will be presented with pairs of abstract stimuli and will learn through trial and error which cue is more likely to yield a reward. For each pair of stimuli, participants will be instructed to select one stimulus by pressing a grip force device for 3 consecutive seconds. They will hold one grip force device in each hand and are instructed to press the grip force corresponding to the side of the selected stimulus.

To provide varying levels of difficulty, the win probabilities of the "correct" stimulus will differ between easy and hard trials. In easy trials, the win probability of the correct stimulus will be 0.75, meaning that choosing the associated action will result in a reward 75% of the time. In hard trials, the win probability of the correct stimulus will be slightly lower at 0.65. The win probability of the other stimulus (the "incorrect" stimulus) will be calculated as 1 minus the probability of the correct stimulus.

The task will consist of eight cue pairs divided into four blocks. Each block contains two pairs, one with a high difficulty and one with a low difficulty. During two blocks, participants will be stimulated with high-intensity stimulation whenever they receive a reward, regardless of the chosen action. During the other two blocks, stimulation will be administered whenever participants perform the action associated with the correct stimulus (the cue more likely to give a reward), regardless of the outcome. As an additional control condition in addition to sham, the intensity will be varied between low-intensity taVNS that is insufficient to induce vagal activation (constant between all participants) and high-intensity stimulation as determined by individual calibration. For each trial, the correct choice will receive high-intensity stimulation, while the incorrect choice will be paired with low-intensity stimulation.