Muscle DamageNCT04733911
Recovery Kinetics Following Eccentric Exercise
A study testing 75 eccentric contractions, 150 eccentric contractions and 300 eccentric contractions in people with Muscle Damage, run by University of Thessaly at 1 site in Greece.
Trial summary
The aim of this study will be to investigate the recovery kinetics of neuromuscular fatigue, muscle microtrauma indicators, performance and oxidative stress factors after three different exercise induced muscle damage (EIMD) protocols in adult Males. Read more about this study
- Who is it for?
- Ages 18 to 30 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Muscle Damage
- What is being tested?
- 75 eccentric contractions, 150 eccentric contractions and 300 eccentric contractions
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 10 participants
- Where
- 1 siteGreece
- Study dates
- 1 Apr 2021to 30 Apr 2022
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 30 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Aged between 18 and 30 years
- Body Mass Index between 18.5-24.9 kg/m*m
- Free of chronic diseases
- Free of musculoskeletal injury
- participants should be non-smokers
Who cannot join
- Musculoskeletal injury
- Chronic disease
- Use of alcohol, caffeine and any type of nutritional supplements or medication before (≥ 6 months) and throughout the study.
Trial locations and contacts
1 site in 1 country.
Greece1 site
University o Thessaly, School of Physical Education and Sports Science
Trikala, 42100
About this study
Official title: Recovery Kinetics Following Three Exercise-induced Muscle Damage Protocols in Adult Males
The aim of this study will be to investigate the recovery kinetics of neuromuscular fatigue, muscle microtrauma indicators, performance and oxidative stress factors after three different exercise induced muscle damage (EIMD) protocols in adult Males. Also, this study will determine the comparison among three different eccentric protocols in neuromuscular fatigue, muscle microtrauma indicators, performance and oxidative stress factors. The eccentric protocols will be differentiated in training volume and specifically in the eccentric repetitions in isokinetic dynamometer.
Read the detailed description
It is known that eccentric exercise induces muscle microtrauma. Also, eccentric type of exercise are associated with inflammatory response. The leukocytes and the immune cells during the phagocytosis process alter the redox status causing secondary muscle damage to the muscle tissue and also increase oxidative stress concentration. Despite of the existence of studies that examine the muscle damage indicators, inflammatory response, performance and neuromuscular fatigue and oxidative stress concentrations after eccentric exercise, however no studies are detected in order to compare the differences among three eccentric protocols. The eccentric protocols will be differentiated in training volume and specifically in the eccentric repetitions in isokinetic dynamometer. Specifically, a randomized, fourth-trial, cross-over, repeated measures design will be applied. Healthy male adults (age 18-30 years) will participate in the present study. Also, it is considered necessary that the participants will not suffer from any musculoskeletal injuries that will limit their ability to perform the exercise protocols. Also the participants will not be smokers and will not consume alcohol and nutritional supplements.
In the first phase all participants will sign an informed consent form after they will be informed about all benefits and risks of this study and they will sign a recent historical of musculoskeletal injury or illness form. Subsequently, fasting blood samples will be collected by venipuncture using a disposable needle (20-gauge) in order to measure muscle damage markers (CK), inflammation markers (WBC) and oxidative stress markers (TBARS, PC, GSH, ΤΑC, CAT, UA). After, delayed onset muscle soreness (DOMS) in the knee flexors (KF) and extensors (KE) of both limbs, body weight (BW), height and body composition (DXA method) will be measured in the lab. Completing the first phase, participants will be instructed by a dietitian how to record a 5 days diet recalls estimating the energy intake during the trials will be the same. The knee range of motion (KJRM) will be determined by the use of a goniometer, 24 hours after (second phase). The counter movement jump (CMJ) will be evaluated on a force platform using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the data for each leg. During the CMJ will be measured the jump height (cm), the ground reaction force (N), the peak and mean power (W/kg), the vertical stiffness (Kvert, N/m/kg) and the peak rate of force development (RFD, N/s), while at the same time will be evaluated the change in peak and mean normalized EMG during the eccentric and concentric phases of the counter movement jump, for the vastus medialis (VM) and vastus lateralis muscles. Electromyography data will be collected wirelessly at 2.000 Hz using a Myon MA-320 EMG system. The peak eccentric and concentric isokinetic torque of the knee flexors and extensors, in both limbs will be evaluated on an isokinetic dynamometer at 60°/sec. Also, the isometric peak torque of the knee extensors will be evaluated at 65° in both limbs. Finally, Open-circuit spirometry will be utilized for assessment of maximal oxygen consumption (VO2max) using an automated online pulmonary gas exchange system via breath-by-breath analysis during a graded exercise testing on a treadmill.
96 hours after, the participants are going to perform one of the three eccentric protocols randomly, on an isokinetic dynamometer. The eccentric protocol will be performed on a different limb for each trial. The ECC 75 trial will include 75 eccentric maximum repetitions (5 sets, 15 reps/set and recovery period: 30sec.) at 60°/sec. The ECC 150 trial will include 150 eccentric maximum repetitions (10 sets, 15 reps/set and recovery period: 30sec.) at 60°/sec. The ECC 300 trial will include 300 eccentric maximum repetitions (20 sets, 15 reps/set and recovery period: 30sec.) at 60°/sec. The DOMS indicator in the knee flexors (KF) and extensors (KE) of both limbs and the KJRM will be evaluated immediately after each protocol. Also, the (CMJ) will be evaluated on a force platform using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the data for each leg. During the CMJ will be measured the jump height (cm), the ground reaction force (N), the peak and mean power (W/kg), the vertical stiffness (Kvert, N/m/kg) and the peak rate of force development (RFD, N/s), while at the same time will be evaluated the change in peak and mean normalized EMG during the eccentric and concentric phases of the counter movement jump, for the vastus medialis (VM) and vastus lateralis muscles. Electromyography data will be collected wirelessly at 2.000 Hz using a Myon MA-320 EMG system. In addition, the peak eccentric and concentric isokinetic torque of the knee flexors and extensors, in both limbs will be evaluated on an isokinetic dynamometer at 60o/sec. Also, the isometric peak torque of the knee extensors will be evaluated at 65o in both limbs. All the above markers will be evaluated 24, 48 and 192 hours post eccentric protocol, however at these time points, fasting blood samples (20ml) will be collected in order to estimate hematological and biochemical indicators. Α 2 - week washout period will be adapted among trials. After, the participants will perform the process until they complete the trials.
- Conditions
- Muscle Damage
- Being tested
- 75 eccentric contractions, 150 eccentric contractions, 300 eccentric contractions
- Keywords
- muscle damage, oxidative stress, Eccentric exercise, Neuromuscular fatigue, Recovery
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
75 eccentric contractionsOther
An acute bout of eccentric exercise will be performed on an isokinetic dynamometer, including 5 sets of 15 repetitions, with 30 sec rest intervals,at 60 degrees/sec.
150 eccentric contractionsOther
An acute bout of eccentric exercise will be performed on an isokinetic dynamometer, including 10 sets of 15 repetitions, with 30 sec rest intervals,at 60 degrees/sec.
300 eccentric contractionsOther
An acute bout of eccentric exercise will be performed on an isokinetic dynamometer, including 20 sets of 15 repetitions, with 30 sec rest intervals,at 60 degrees/sec.
| Study group | Role | What they receive |
|---|---|---|
| Eccentric 75 | Experimental | Participants in this arm will perform an eccentric exercise protocol consisted of 75 eccentric repetetions on an isokinetic dynamometer. Receives: Other: 75 eccentric contractions |
| Eccentric 150 | Experimental | Participants in this arm will perform an eccentric exercise protocol consisted of 150 eccentric repetetions on an isokinetic dynamometer. Receives: Other: 150 eccentric contractions |
| Eccentric 300 | Experimental | Participants in this arm will perform an eccentric exercise protocol consisted of 300 eccentric repetetions on an isokinetic dynamometer. Receives: Other: 300 eccentric contractions |
| Control | No intervention | Participants in this arm will receive no intervention. |
What is being measured
The primary outcome is the main question the study is designed to answer.
Change in white blood cell count
White blood cell count will be measured using an automatic blood analyzer
Measured: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in catalase activity
Concentration of catalase will be measured in red blood cells
Measured: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in uric acid in plasma
concentration of uric acid will be measured in plasma
Measured: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in protein carbonyls in blood
Concentration of protein carbonyls will be measured in red blood cells
Measured: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in total antioxidant capacity
total antioxidant capacity will be measured in serum
Measured: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in reduced glutathione in blood
Concentration of oxidized glutathione will be measured in red blood cells
Measured: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in thiobarbituric acid reactive substances
Thiobarbituric acid will be measured in plasma
Measured: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in Creatine kinase in blood
Creatine kinase will be measured in plasma
Measured: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in countermovement jump height
Countermovement jump height will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the jump height data for each leg.
Measured: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
Change in ground reaction force (GRF) during countermovement jump test
The ground reaction force will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the GRF data for each leg.
Measured: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
Change in peak power during countermovement jump test
The peak power will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the peak power data for each leg.
Measured: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
Change in mean power during countermovement jump test
The mean power will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the mean power data for each leg.
Measured: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
Change in vertical stifness during countermovement jump test
The vertical stiffness will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of vertical stifness data for each leg.
Measured: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
Change in peak rate of force develpemnt during countermovement jump test
The Peak rate of force development will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the Peak RFD data for each leg.
Measured: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
Change in peak normalized EMG during the eccentric and concentric phases of the counter movement jump
Electromyography data will be collected wirelessly at 2.000 Hz using a Myon MA-320 EMG system (Myon AG, Schwarzenberg, Switzerland) for the vastus medialis (VM) and vastus lateralis muscles.
Measured: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
Change in mean normalized EMG during the eccentric and concentric phases of the counter movement jump
Electromyography data will be collected wirelessly at 2.000 Hz using a Myon MA-320 EMG system (Myon AG, Schwarzenberg, Switzerland) for the vastus medialis (VM) and vastus lateralis muscles.
Measured: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
Change in knee range of motion
Knee range of motion will be measured by goniometer
Measured: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
Change in eccentric isokinetic knee extensors peak torque
Eccentric isokinetic knee extensors peak torque will be assessed on an isokinetic dynamometer
Measured: At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in concentric isokinetic knee extensors peak torque
Concentric isokinetic knee extensors peak torque will be assessed on an isokinetic dynamometer
Measured: At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in eccentric isokinetic knee flexors peak torque
Eccentric isokinetic knee flexors peak torque will be assessed on an isokinetic dynamometer
Measured: At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in concentric isokinetic knee flexors peak torque
Concentric Iisokinetic knee flexors peak torque will be assessed on an isokinetic dynamometer
Measured: At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in isometric peak torque
The isometric peak torque will be assessed on an isokinetic dyanmometer
Measured: At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in fatigue index of isometric torque during 10 second
Fatigue rate during MVIC will be estimated through the percent drop of peak torque between the first and the last three seconds of a 10-secong maximal isometric contraction.
Measured: At baseline, 1hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
Change in delayed onset of muscle soreness (DOMS) in the knee flexors (KF) and extensors (KE) of both limbs
Muscle soreness (KF and KE) will be assessed during palpation of the muscle belly and the distal region
Measured: At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
Secondary outcomes (5)
Body weight
Body weight will be measured on a beam balance with stadiometer
Measured: At baseline
Body height
Body height will be measured on a beam balance with stadiometer
Measured: At baseline
Body fat
Body fat will be measured by using Dual-emission X-ray absorptiometry
Measured: At baseline
Dietary intake
Dietary intake will be assessed using 5 -day diet recalls
Measured: Over a 5 -day period at baseline
Maximal oxygen consumption (VO2max)
Maximal oxygen consumption will be measured by open circuit spirometry via breath by breath method
Measured: At baseline
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted2 Feb 2021
- Study start1 Apr 2021
- Primary completion28 Feb 2022
- Study completion30 Apr 2022
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Muscle DamageStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- None (open label)
- Enrolment
- 10 participants (actual)
Sponsor and investigators
- Lead sponsor
- University of Thessaly
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Ioannis G. Fatouros, University of Thessaly
- Investigators
- Ioannis G Fatouros, Prof, Study director, University of Thessaly
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT04733911
- Sponsor's protocol ID
- Recovery-Eccentrics-UTH
- Status last verified
- Jun 2022
- First submitted
- 25 Jan 2021
- Last updated
- 10 Jun 2022
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
Study at a glance
From the registry record, last updated by the sponsor on 10 Jun 2022. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Recovery Kinetics Following Three Exercise-induced Muscle Damage Protocols in Adult Males
- ClinicalTrials.gov ID
- NCT04733911
- Acronym / sponsor ID
- Recovery-Eccentrics-UTH
- Conditions
- Muscle Damage
- Keywords
- muscle damage, oxidative stress, Eccentric exercise, Neuromuscular fatigue, Recovery
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 10 participants (actual)
- Lead sponsor
- University of ThessalyUniversity, hospital or other
- Study start
- 1 Apr 2021 (actual)
- Primary completion
- 28 Feb 2022 (actual)
- Study completion
- 30 Apr 2022 (actual)
- First posted
- 2 Feb 2021 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The aim of this study will be to investigate the recovery kinetics of neuromuscular fatigue, muscle microtrauma indicators, performance and oxidative stress factors after three different exercise induced muscle damage (EIMD) protocols in adult Males. Also, this study will determine the comparison among three different eccentric protocols in neuromuscular fatigue, muscle microtrauma indicators, performance and oxidative stress factors. The eccentric protocols will be differentiated in training volume and specifically in the eccentric repetitions in isokinetic dynamometer.
Detailed description
It is known that eccentric exercise induces muscle microtrauma. Also, eccentric type of exercise are associated with inflammatory response. The leukocytes and the immune cells during the phagocytosis process alter the redox status causing secondary muscle damage to the muscle tissue and also increase oxidative stress concentration. Despite of the existence of studies that examine the muscle damage indicators, inflammatory response, performance and neuromuscular fatigue and oxidative stress concentrations after eccentric exercise, however no studies are detected in order to compare the differences among three eccentric protocols. The eccentric protocols will be differentiated in training volume and specifically in the eccentric repetitions in isokinetic dynamometer. Specifically, a randomized, fourth-trial, cross-over, repeated measures design will be applied. Healthy male adults (age 18-30 years) will participate in the present study. Also, it is considered necessary that the participants will not suffer from any musculoskeletal injuries that will limit their ability to perform the exercise protocols. Also the participants will not be smokers and will not consume alcohol and nutritional supplements.
In the first phase all participants will sign an informed consent form after they will be informed about all benefits and risks of this study and they will sign a recent historical of musculoskeletal injury or illness form. Subsequently, fasting blood samples will be collected by venipuncture using a disposable needle (20-gauge) in order to measure muscle damage markers (CK), inflammation markers (WBC) and oxidative stress markers (TBARS, PC, GSH, ΤΑC, CAT, UA). After, delayed onset muscle soreness (DOMS) in the knee flexors (KF) and extensors (KE) of both limbs, body weight (BW), height and body composition (DXA method) will be measured in the lab. Completing the first phase, participants will be instructed by a dietitian how to record a 5 days diet recalls estimating the energy intake during the trials will be the same. The knee range of motion (KJRM) will be determined by the use of a goniometer, 24 hours after (second phase). The counter movement jump (CMJ) will be evaluated on a force platform using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the data for each leg. During the CMJ will be measured the jump height (cm), the ground reaction force (N), the peak and mean power (W/kg), the vertical stiffness (Kvert, N/m/kg) and the peak rate of force development (RFD, N/s), while at the same time will be evaluated the change in peak and mean normalized EMG during the eccentric and concentric phases of the counter movement jump, for the vastus medialis (VM) and vastus lateralis muscles. Electromyography data will be collected wirelessly at 2.000 Hz using a Myon MA-320 EMG system. The peak eccentric and concentric isokinetic torque of the knee flexors and extensors, in both limbs will be evaluated on an isokinetic dynamometer at 60°/sec. Also, the isometric peak torque of the knee extensors will be evaluated at 65° in both limbs. Finally, Open-circuit spirometry will be utilized for assessment of maximal oxygen consumption (VO2max) using an automated online pulmonary gas exchange system via breath-by-breath analysis during a graded exercise testing on a treadmill.
96 hours after, the participants are going to perform one of the three eccentric protocols randomly, on an isokinetic dynamometer. The eccentric protocol will be performed on a different limb for each trial. The ECC 75 trial will include 75 eccentric maximum repetitions (5 sets, 15 reps/set and recovery period: 30sec.) at 60°/sec. The ECC 150 trial will include 150 eccentric maximum repetitions (10 sets, 15 reps/set and recovery period: 30sec.) at 60°/sec. The ECC 300 trial will include 300 eccentric maximum repetitions (20 sets, 15 reps/set and recovery period: 30sec.) at 60°/sec. The DOMS indicator in the knee flexors (KF) and extensors (KE) of both limbs and the KJRM will be evaluated immediately after each protocol. Also, the (CMJ) will be evaluated on a force platform using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the data for each leg. During the CMJ will be measured the jump height (cm), the ground reaction force (N), the peak and mean power (W/kg), the vertical stiffness (Kvert, N/m/kg) and the peak rate of force development (RFD, N/s), while at the same time will be evaluated the change in peak and mean normalized EMG during the eccentric and concentric phases of the counter movement jump, for the vastus medialis (VM) and vastus lateralis muscles. Electromyography data will be collected wirelessly at 2.000 Hz using a Myon MA-320 EMG system. In addition, the peak eccentric and concentric isokinetic torque of the knee flexors and extensors, in both limbs will be evaluated on an isokinetic dynamometer at 60o/sec. Also, the isometric peak torque of the knee extensors will be evaluated at 65o in both limbs. All the above markers will be evaluated 24, 48 and 192 hours post eccentric protocol, however at these time points, fasting blood samples (20ml) will be collected in order to estimate hematological and biochemical indicators. Α 2 - week washout period will be adapted among trials. After, the participants will perform the process until they complete the trials.
Study design
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- None (open label)
- Number of arms
- 4
| Arm | Type | Receives |
|---|---|---|
| Eccentric 75 | Experimental | Other: 75 eccentric contractionsParticipants in this arm will perform an eccentric exercise protocol consisted of 75 eccentric repetetions on an isokinetic dynamometer. |
| Eccentric 150 | Experimental | Other: 150 eccentric contractionsParticipants in this arm will perform an eccentric exercise protocol consisted of 150 eccentric repetetions on an isokinetic dynamometer. |
| Eccentric 300 | Experimental | Other: 300 eccentric contractionsParticipants in this arm will perform an eccentric exercise protocol consisted of 300 eccentric repetetions on an isokinetic dynamometer. |
| Control | No intervention | –Participants in this arm will receive no intervention. |
Who may qualify
- Ages
- 18 to 30 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (5 listed)
- Aged between 18 and 30 years
- Body Mass Index between 18.5-24.9 kg/m*m
- Free of chronic diseases
- Free of musculoskeletal injury
- participants should be non-smokers
Key exclusion criteria (3 listed)
- Musculoskeletal injury
- Chronic disease
- Use of alcohol, caffeine and any type of nutritional supplements or medication before (≥ 6 months) and throughout the study.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| 75 eccentric contractionsAn acute bout of eccentric exercise will be performed on an isokinetic dynamometer, including 5 sets of 15 repetitions, with 30 sec rest intervals,at 60 degrees/sec. | Other | Eccentric 75 |
| 150 eccentric contractionsAn acute bout of eccentric exercise will be performed on an isokinetic dynamometer, including 10 sets of 15 repetitions, with 30 sec rest intervals,at 60 degrees/sec. | Other | Eccentric 150 |
| 300 eccentric contractionsAn acute bout of eccentric exercise will be performed on an isokinetic dynamometer, including 20 sets of 15 repetitions, with 30 sec rest intervals,at 60 degrees/sec. | Other | Eccentric 300 |
Primary outcome
Change in white blood cell count
Time frame: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
White blood cell count will be measured using an automatic blood analyzer
Change in catalase activity
Time frame: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
Concentration of catalase will be measured in red blood cells
Change in uric acid in plasma
Time frame: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
concentration of uric acid will be measured in plasma
Change in protein carbonyls in blood
Time frame: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
Concentration of protein carbonyls will be measured in red blood cells
Change in total antioxidant capacity
Time frame: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
total antioxidant capacity will be measured in serum
Change in reduced glutathione in blood
Time frame: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
Concentration of oxidized glutathione will be measured in red blood cells
Change in thiobarbituric acid reactive substances
Time frame: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
Thiobarbituric acid will be measured in plasma
Change in Creatine kinase in blood
Time frame: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.
Creatine kinase will be measured in plasma
Change in countermovement jump height
Time frame: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
Countermovement jump height will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the jump height data for each leg.
Change in ground reaction force (GRF) during countermovement jump test
Time frame: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
The ground reaction force will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the GRF data for each leg.
Change in peak power during countermovement jump test
Time frame: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
The peak power will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the peak power data for each leg.
Change in mean power during countermovement jump test
Time frame: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
The mean power will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the mean power data for each leg.
Change in vertical stifness during countermovement jump test
Time frame: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
The vertical stiffness will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of vertical stifness data for each leg.
Change in peak rate of force develpemnt during countermovement jump test
Time frame: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
The Peak rate of force development will be measured using two force platforms at 1000 Hz, with each foot in parallel on the two platforms providing a separate yet time-synchronized measurement of the Peak RFD data for each leg.
Change in peak normalized EMG during the eccentric and concentric phases of the counter movement jump
Time frame: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
Electromyography data will be collected wirelessly at 2.000 Hz using a Myon MA-320 EMG system (Myon AG, Schwarzenberg, Switzerland) for the vastus medialis (VM) and vastus lateralis muscles.
Change in mean normalized EMG during the eccentric and concentric phases of the counter movement jump
Time frame: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
Electromyography data will be collected wirelessly at 2.000 Hz using a Myon MA-320 EMG system (Myon AG, Schwarzenberg, Switzerland) for the vastus medialis (VM) and vastus lateralis muscles.
Change in knee range of motion
Time frame: At baseline, at 1 hour and 24, 48 and 192 hours following the eccentric exercise protocol.
Knee range of motion will be measured by goniometer
Change in eccentric isokinetic knee extensors peak torque
Time frame: At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
Eccentric isokinetic knee extensors peak torque will be assessed on an isokinetic dynamometer
Change in concentric isokinetic knee extensors peak torque
Time frame: At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
Concentric isokinetic knee extensors peak torque will be assessed on an isokinetic dynamometer
Change in eccentric isokinetic knee flexors peak torque
Time frame: At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
Eccentric isokinetic knee flexors peak torque will be assessed on an isokinetic dynamometer
Change in concentric isokinetic knee flexors peak torque
Time frame: At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
Concentric Iisokinetic knee flexors peak torque will be assessed on an isokinetic dynamometer
Change in isometric peak torque
Time frame: At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
The isometric peak torque will be assessed on an isokinetic dyanmometer
Change in fatigue index of isometric torque during 10 second
Time frame: At baseline, 1hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
Fatigue rate during MVIC will be estimated through the percent drop of peak torque between the first and the last three seconds of a 10-secong maximal isometric contraction.
Change in delayed onset of muscle soreness (DOMS) in the knee flexors (KF) and extensors (KE) of both limbs
Time frame: At baseline, 1 hour, at 24, 48 and 192 hours following the eccentric exercise protocol.
Muscle soreness (KF and KE) will be assessed during palpation of the muscle belly and the distal region
5 secondary outcomes
Body weight
Time frame: At baseline
Body height
Time frame: At baseline
Body fat
Time frame: At baseline
Dietary intake
Time frame: Over a 5 -day period at baseline
Maximal oxygen consumption (VO2max)
Time frame: At baseline
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 10 Jun 2022.
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Evaluating Long-term Use of a Pediatric Robotic Exoskeleton (P.REX/Agilik) to Improve Gait in Children With Movement Disorders
Cerebral Palsy, Muscular Dystrophy
Not yet recruiting
Effects of Manual Lymphatic Drainage and Pressotherapy on Muscle Recovery After Eccentric Exercise in Physically Active Young Adults
Young Athletes
RecruitingPhase 4
Ramipril Treatment of Claudication: Oxidative Damage and Muscle Fibrosis
Peripheral Arterial Disease
Recruiting
Acute Cryotherapy on Musculoskeletal Function and Biomarkers
Exercise Induced Muscle Damage, Cryotherapy
Recruiting
Mineral-Infused Clothing for Recovery From Muscle Soreness and Muscle Damage After Exercise
Delayed Onset Muscle Soreness, Exercise-Induced Muscle Damage
Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
Ioannis G Fatouros, Prof study director
University of Thessaly
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing 75 eccentric contractions, 150 eccentric contractions and 300 eccentric contractions in people with Muscle Damage, run by University of Thessaly at 1 site in Greece.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 5 inclusion and 3 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 10 Jun 2022. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Greece. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Change in white blood cell count (time frame: At baseline, at 24, 48 and 192 hours following the eccentric exercise protocol.). The study lists 24 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 10 Jun 2022.