Novel Molecular Spectrometric Biomarkers in Blood Plasma as an Early Diagnostic Tool in HCC

Subjects: Patient recruitment will be performed at the Department of Gastroenterology, Hepatology and Metabolism at the Internal Clinic of the First Faculty of Medicine of the Charles University and the Military University Hospital in Prague (MUH Prague). The selection of sample set sizes is clarified below (subsection Data collection and statistical analysis).

Group A: ~100 patients with liver cirrhosis with the HCC diagnosis confirmed according to standard diagnostic criteria published recently by European Association for the study of the liver (EASL). Patients of all HCC stages according to Barcelona Clinic Liver Cancer (BCLC), in our department ~40-50 patients per year, will be included.

Group B: ~100 patients with liver cirrhosis without HCC or dysplastic nodules at the time of examination and within the next 12 months of follow-up. The patients will be monitored by regular clinical visits with general laboratory exams and liver ultrasound in 6-month intervals (recommended surveillance for patients with liver cirrhosis in risk of HCC development).

Group C: ~50 healthy volunteers without liver cirrhosis (Czech Army recruits, Czech Army active military personnel).

Routinely examined biochemistry (e.g. total protein, albumin, alpha feto protein, etc.) of all studied groups will be correlated with the newly identified spectroscopic/metabolomic biomarkers. A signed informed consent will be secured from each subject included in the study (Inclusion/exclusion criteria listed below). Plasma preparation: Following the already established procedure for sample collection, preparation and analysis, the whole anticoagulant-treated (K3EDTA) blood of all subjects will be collected by venipuncture and centrifuged at 1500 × g for 10 minutes. The plasma fractions will be frozen immediately (stored at -80 °C) and transported to the UCT Prague for spectroscopic and metabolomics analyses. Prior to each analysis, the samples will be thawed at room temperature (~25 °C) and centrifuged through a 0.45 μm membrane filter at 13,000 × g for 10 minutes. Subsequently, the spectral measurements will be carried out (in the case of ROA and Raman, a kinetic fluorescence quencher will be added to suppress the spectrally broad background of large amplitude, which overlaps the signals of interest and is caused by a high content of fluorescing molecules in blood plasma).

Spectroscopic analysis: To meet the project objectives, the investigators will utilize a unique combination of two chiroptical methods - ECD and ROA - supplemented by non-polarized variants - IR absorption and Raman spectroscopy. While ECD detects the absorption difference between left and right circularly polarized radiation, the commercially available scattered circular polarization ROA setup operates with the small intensity difference between the circularly polarized components in the scattered light using non-polarized incident radiation. Both of these methods exhibit inherent sensitivity to the 3D structure and conformation of several chiral biomolecules, such as peptides, proteins, saccharides, nucleic acids and others (20). Some of these types of compounds might be among those, whose 3D structure or concentration in blood plasma varies during HCC development. While ECD sensitively reflects the overall conformation of chiral molecules, ROA focuses on particular bond types; and thus, reflects rather the structural details. In addition to the peptide-backbone bands from regular secondary structure elements, specifically helices and beta-sheets, the ROA spectra contain distinct bands from loops and turns and, as such; they provide information on the tertiary structure. Therefore, the simultaneous use of ECD, ROA and the non-polarized spectroscopic methods will enhance the reliability of the particular findings.

Moreover, combining different analytical approaches and technologies not yet established in clinical practice will increase the likelihood of detecting minor changes within the spectral features reflecting the conformational and concentration alterations of the HCC-related molecules and/or their metabolites in blood plasma. To the best of our knowledge, our laboratory at the Department of Analytical Chemistry, UCT Prague is the only one in the world to have all of these techniques available, simultaneously having a long-term experience with biofluid analysis. All equipment used for the analyses is commercially available and, thus; may be purchased by clinical laboratories.