Therapy for Children With Neuroblastoma

The study will have five parts or phases. In the first part the combination of irinotecan and gefitinib will be studied. After that, patients who have responded well will have surgery to remove the tumor. This will be followed by a third part which includes about nine months of treatment with cisplatin, adriamycin, etoposide, cyclophosphamide, and topotecan. The fourth part will be intensification with melphalan, etoposide and carboplatin and blood stem cell rescue. During this part, radiation will also be given to the sites of the disease. Finally, monthly treatments with oral retinoic acid, alternating with oral topotecan, will be continued for a total of 16 months of maintenance. It is anticipated that it will take about 2 years to complete this entire treatment plan.

This study has multiple therapeutic, pharmacologic, biologic, and diagnostic imaging objectives:

• To estimate whether oral gefitinib with two courses of intravenous irinotecan will decrease the incidence of fever/neutropenia, duration of hospitalization, duration of intravenous antibiotics and numbers of platelet and RBC transfusions during the first six weeks of treatment compared to the topotecan window in NB97.
• To estimate local control of primary site disease to this treatment plan.
• To estimate the overall survival and progression-free survival in patients treated with this approach.
• To estimate the feasibility of resecting the primary tumor after two courses of irinotecan and gefitinib.
• To evaluate the disposition of irinotecan and gefitinib in previously untreated patients with neuroblastoma.
• To evaluate the disposition of intravenous and oral topotecan in previously untreated patients with neuroblastoma.
• To evaluate the pharmacogenetic determinants of gefitinib and irinotecan pharmacokinetics and pharmacodynamics (e.g., CYP3A4/5, UGT1A1, and ABCG2 (BCRP) polymorphisms).
• To evaluate the pharmacogenetic determinants of topotecan pharmacokinetics and pharmacodynamics (e.g., CYP3A4/5 and ABC transporter polymorphisms).
• To evaluate in tumor samples the molecular and cellular expression of EGFR, MRP4 and ABCG2 (BCRP) utilizing appropriate laboratory techniques.
• To describe the relative frequency of positive bone marrow by sensitive MRD methods at diagnosis, after window therapy, at the time of stem cell harvest, and at several time points following the completion of intensification. These results will be compared with timing and pattern of disease recurrence.
• To describe what percentage of primary or metastatic neuroblastomas have amplified ICAM-2 and chromosome 17.
• To generate preliminary data regarding the potential use of ICAM-2 copy number as a prognostic indicator in neuroblastoma.
• To determine the levels of the angiogenic factors VEGF and bFGF in the peripheral circulation of patients at diagnosis, after window therapy, at the time of stem cell harvest and at several time points following the completion of intensification. These results will be compared with the degree of tumor response and the timing of disease recurrence.
• To procure tumor samples for construction of tissue microarray blocks that will be utilized in further biologic characterization of these tumors.
• To prospectively evaluate FDG PET imaging as a marker of disease at diagnosis, (pre and post window therapy), prior to intensification, and at the completion of therapy.
• To generate preliminary data on the use of contrast enhanced ultrasound and magnetic resonance imaging to evaluate changes in tumor vascularity at various timepoints in therapy.

Details of Treatment Interventions:

Window Phase Irinotecan 15mg/m2 daily x 5 days for two weeks with daily oral gefitinib 112.5 mg/m2 daily x 12 days., followed by 9 day rest, then same course repeated. Subjects that respond to window therapy receive the same course again instead of topotecan for Block 2, course 6 (week 21) of induction.

Induction Therapy (following window):

Cyclophosphamide 1.5 gm/m2 daily x 2 I.V. day 1 \& 2 Adriamycin 50 mg/m2 I.V. day 1 only MESNA: 375 mg/m2 I.V. immediately following cyclophosphamide and at 3 and 6 hours post-infusion.

Etoposide: 30 mg/m2 over 30 minutes, followed by etoposide 250 mg/m2/day x 3 days I.V. by continuous infusion (days 2-5), given during induction therapy courses 1, 4, and 7.

Cisplatin 40 mg/m2/day x 5 I.V. over 1 hour (days 1-5) Etoposide 200 mg/m2/day x 3 I.V. over 1 hour (days 2, 3, 4), given during induction courses 2, 5, and 8.

IV topotecan adjusted to AUC 100 ± 20nghr/ml daily x 5 days for two weeks, during courses 3, 6 (for patients that do not respond to window), and 9 of induction.

Intensification:

Melphalan, Etopophos and carboplatin:Day -8, -7, -6, -5: Melphalan 45 mg/m2 IV Day -4: Etopophos 40 mg/kg/day IV Day -4, -3, \& -2: Carboplatin (AUC target 4.1) Day 0- infusion of peripheral blood stem cells previously harvested by pheresis.

Maintenance:

13 cis-retinoic acid and oral topotecan courses:13-cis-retinoic acid 160 mg/m2/day divided into two equal doses given orally BID x 14 days, followed by a 14 day rest. This will be repeated x one. Subjects less or equal to 12 kg will be given 5.33 mg/kg/day divided BID. These courses are alternated with 2 months of oral topotecan once daily for 5 days for 2 consecutive weeks at 1.8 mg/m2/day , or 0.06 mg/kg/day for patients less than 12 months old (total of 10 doses) for a total of 16 courses (four, two-month courses of each).

Radiation therapy : Radiation therapy to the primary and metastatic disease sites will follow peripheral blood stem cell transplant with the exception of any patient requiring emergent radiation. External beam radiotherapy will be delivered to the primary site and select metastatic sites. Radiotherapy is planned to be initiated four weeks following stem cell reinfusion.

Surgery : After recovery from induction and re-evaluation of tumor status, subjects undergo surgery for resection of the primary tumor mass and careful lymph node staging, if surgery was not possible after the irinotecan and ZD1839 window.

Peripheral blood stem cell collection and infusion : After course 3, subjects undergo peripheral blood stem cell (PBSC) harvest. If this is unsuccessful, harvesting will be done with subsequent chemotherapy courses. Subjects are mobilized with filgrastim (10mcg/kg/day). PBSC harvesting will be performed by leukapheresis if possible, bone marrow harvest if not. Stem cells are stored and re-infused after intensification chemotherapy.